各種アクリル製眼内レンズの家兎水晶体上皮培養細胞への影響

Culture of rabbit lens epithelial cells on various types of acrylic intraocular lens

要約　目的：培養した水晶体上皮細胞に各種のアクリル製眼内レンズ（IOL）が及ぼす影響の検討。方法：生後8週の白色家兎から水晶体上皮細胞を採取し，IOL上にこれを播種した。96時間後に細胞の発育を停止させ，ヘマトキシリン・エオジンで染色し，コラーゲン膜上とIOL表面の細胞密度と性状を生体顕微鏡で観察した。アクリル製IOLには，疎水性3種と親水性1種を用いた。結果：親水性IOL表面には細胞付着が少なく，コラーゲン膜上には伸展していた。疎水性IOL表面には多数の細胞が付着し，コラーゲン膜上の伸展が少なかった。細胞の形状は，各IOL間で異なっていた。結論：親水性と疎水性アクリル製IOLでは，その種類により，後発白内障の発生頻度と伸展細胞の性状に特異性がある。

Abstract.　Purpose：To evaluate the effect of different types of acrylic intraocular lens（IOL）on cultured lens epithelial cells. Method：Lens epithelial cells were obtained from white rabbits aged 8 weeks. The cells were sprinkled over IOLs placed in the media. After 96 hours,the cells were stained by hematoxylineosin and were examined with a biomicroscope. Attention was paid to the cell population and cell shape adherent to collagen membrane and IOLs. Four types of IOL were used including hydrophobic and hydrophilic ones. Results：Cells adherent to hydrophilic IOLs were characterized by lesser population and by better sheet formation over collagen membrane. Cells adherent to hydrophobic IOLs were characterized by higher population and by lesser sheet formation over collagen membrane. Cultured cells showed different shapes depending on the type of IOLs. Conclusion：Hydrophilic and hydrophobic IOLs exert differences in cultured cell types and in incidence of secondary posterior capsular opacity.