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Studies on some problems concerning enzymatic zonulolysis Isao Takaku 1 , Suguru Fukushi 1 , Nobuhiro Abe 1 , Kunio Sekiguchi 1 , Haruki Watanabe 1 , Ai Ishigaki 1 , Yoneshige Ikeda 1 1Department of Ophthalmology, School of Medicine, Tohoku University pp.407-414
Published Date 1962/4/15
DOI https://doi.org/10.11477/mf.1410202474
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PART I. BIOCHEMICAL STUDIES ON THE ENZYMATICAL INFLUENCE OF α-CHY-MOTRYPSIN ON THE OCULAR TISSUES.

The effect of α-chymotrypsin on the ocular tissues is reported. Each tissue of the cattle eye separated to the cornea, the iris, the lens, the anterior hyaloid membrane, the chorioid, the retina and the vitreous filtrate, was homogenized with a certain volume of Borate Buffer (pH 8.2). Two ml of each homogenate was incubated at 35℃ for 60 min. with 0.5 ml of α-chymotrypsin solutions (containing 0.3 mg of Zonolysin, Mochida). The digestion was stopped after adding 2 ml of 0.44 M.T.C.A.


Copyright © 1962, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1308 印刷版ISSN 0370-5579 医学書院

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