桐沢型ぶどう膜炎患者眼内液を用いた経時的ウイルス学的検索

Virological analysis of intraocular fluids over time in acute retinal necrosis

（26C1-9）　桐沢型ぶどう膜炎患者23例の眼内液（前房水22検体，硝子体液17検体）について，検体採取時期がpolymerase chain reaction（PCR）法および抗体率の結果に与える影響を検討した。前房水を対象とした検討結果では，PCR法陽性群および抗体率陰性群は有意に早期に検体採取が行われていた。一方，硝子体液を対象とした場合，PCR法，抗体率ともに陰性群と陽性群の間に採取時期の有意差はなかった。PCR法，抗体率の陽生率に着目すると，発症後20日以前に採取した検体ではPCR法が，20日を過ぎると抗体率の陽性率が高く，発症早期にはPCR法を，日数の経過した症例では抗体率を第一選択にすることが有効な検体利用法であると考えた。

We tested intraocular fluids in 23 cases of acute retinal necrosis regarding viral antibody titer and polymerase chain reaction (PCR). Aqueous humor was obtained in 22 samples and vitreous aspirate in 17. Aqueous sample obtained during the early phase of the disease showed positive PCR identification of the causative virus but unremarkable viral antibody ratio. Vitreous samples showed similar results in the incidence of positive PCR results and viral antibody ratio regardless the timing of sampling. As an overall finding, samples taken before 20 days after onset of disease showed higher rates of positive PCR findings. Samples taken thereafter showed higher incidence of viral antibody titer. The findings show that PCR studies are indicated for the early stage of the disease and that viral antibody studies are more sensitive for later stages.