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Laser flare-cell meter : A second prototype Mitsuru Sawa 1 , Yutaka Tsurimaki 1 , Tadahiko Tsuru 1 , Hiroyuki Shimizu 1 1Dept of Ophthalmol, Jichi Med Sch pp.660-664
Published Date 1988/6/15
DOI https://doi.org/10.11477/mf.1410210414

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  • Abstract
  • Look Inside

We developed an instrument for in vivo quantita-tion of protein concentration and cells in the ante-rior chamber. It consists of three main compo-nents : illumination, measuring device and data processing system.

For the illumination, an He-Ne laser beam is used, with the size of 7×10μm at the place of focus. A photon-counting photomultiplier measures the intensity of scattered beam in the aqueous through a sampling window 0.3×0.5 mm in size. A personal computer controls the system and ana-lyzes the detected signals.

The system has dual modes for measurement of protein concentration and the number of cells. In protein concentration mode, the laser beam is set at 13μW in power output to scan vertically for 0.6 mm. It takes 1 second for the measurement. In cell -count mode, the laser beam is set at 78μW to scan two-dimensionally, 0.6×0.3 mm.It takes 1.2 secondsfor the measurement.

We found a good correlation between the thus measured value and the actual one using human plasma (r=0.993, p=0.00). We also confirmed a good correlation regarding the cell counting mode between the measured value and slit-lamp micro-scopic findings in senile subjects.

In normal eyes in young subjects, values for protein concentration measured 0.13ア0.056 (meanアSD/msec) under mydriatics and 0.15+0. 046 without, corresponding to 3.33 and 3.85 mg/dl. The difference was not significant. Eyes in senile subjects showed significantly higher values, 0.33+0. 15 (p<0.05).

The present system promises to be of clinical value in quantitative measurement of protein con-centration and cells in the anterior chamber when further refined.

Rinsho Ganka (Jpn J Clin Ophthalmol) 42(6) : 660-664, 1988


Copyright © 1988, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1308 印刷版ISSN 0370-5579 医学書院

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