ポーラログラフィーならびに濾紙電気泳動法による血清蛋白質の季節的変動

SEASONAL CHANGES IN SERUM PROTEINS STUDIED BY POLAROGRAPHY AND FILTER-PAPER ELECTROPHORESIS

緒言

　我々は，ポーラログラフィーならびに濾紙電気泳動法を用い，精神疾患の血清蛋白質について研究を行なつたが，その一部として季節的変動の問題を検討した。およそ生体の血液成分について検索する場合に，自然環境の影響は，当然考慮せねばならぬ条件のひとつであり，ことに長期にわたつて精神疾患の経過を追つて検査する際にはぜひともその間の季節による血液成分の変動をあらかじめ明らかにしておく必要がある。よつて各季節ごとに正常群と精神分裂病群とをあわせ検査して，それらの季節的な変動を検索しさらに正常群と分裂病群との差異をも比較検討した。

For purposes of investigating seasonal chan-ges in serum protein, we performed native, denaturation and filtrate tests by polarography and examined protein, glycoprotein and lipo-protein fractions in every season by filterpaper electrophoresis in 56 normal subjects and 61 cases of old schizophrenia.

The following results were obtained:

1) The amplitude of a polarographic native wave showed a definite decrease in both nor-mal and schizophrenic groups in winter. The amplitude of a denaturation wave tended to decrease in both groups in winter, whereas it had a tendency to increase somewhat in spring. In a normal groups tendency toward a greater increase of the amplitude of the denaturation wave in the spring was observed than in the schizophrenic group. Accordingly lowered SH-group activities were recognized in winter in both groups.

2) In the filtrate test both normal and schizophrenic groups exhibited tendencies to-ward an increased aplitude of the wave in spring. Especially in the normal group this increase was apparently greater than in win-ters. The schizophrenic group, when com-pared with the normal group, showed the greater tendency of filtrate wave amplitude in each season. Therefore the high tendency of the activity of the SH-group in mucoprotein was observed in both groups.

3) Speaking of changes in protein fractions studied by electrophoresis, both normal and schizophrenic groups showed an increase in the albumin fraction in summer. In summer there was a decrease in α₂-, β- and γ- globulin fractions in the normal group and the γ- globulin fraction it the schizophrenic group. The normal group exhibited a slight increase in the α₁- globulin fraction. The albumin fraction in the normal group tended to be greater in every season than in the schizophrenic group, whereas the β- and γ- globulin fractions had a tendency to be greater in tne schizophrenic, than in the normal group.

4) Concerning glycoprotein, both normal and schizophrenic groups showed a decrease in the albumin fraction in winter. In the nor-mal group there was an increase in the γ-globulin fraction in winter. In each season the β- globulin fraction in the schizophrenic group tended to be greater than in the normal group.

5) No significant seasonal changes were seen in lipoprotein fractions.

6) Emphasis was placed on the fact that these seasonal changes are finidings that should be considered in prolonged studies, and the mechanisms responsible for these changes were discussed.