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Function of the electron transfer system in mitochondria. Takayuki OZAWA 1 , Masashi TANAKA 1 , Morimitsu NISHIKIMI 1 , Hiroshi Suzuki 1 1Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya pp.579-591
Published Date 1987/8/10
DOI https://doi.org/10.11477/mf.1431905909
  • Abstract
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The structural basis for the energy transduction in mitochondria was studied by multiple approa-ches. Firstly, the electron-transfer complexes were isolated from beef heart mitochondria, highly purified by hydrophobic chromatography and affi-nity chromatography, and crystallized into three dimensional crystals. Their structures were studied by electron microscopy and electron diffraction. Secondly, the complexes were resolved into their subunits and reconstituted from the components.Namely, the iron-sulfur protein, cytochrome b, cytocrome c1, ubiquinone-binding protein, and two core proteins were isolated from Complex III, and a part of its electron-transfer pathway was reconstituted from the components. We also isolated the ubiquinone-binding protein from Complex I, and characterized its function in electron transport and energy transduction. The antibodies raised against the complexes or the subunits were used to clone the human cDNA for cytochrome c1. The antibodies were also useful for analyzing the abnormality of subunit composition of the complexes in patients with mitochondrial cytopa-thies whose mitochondrial energy transduction was impaired. The molecular architecture of the mitochondria in the normal or pathological state would be elucidated by these approaches.


Copyright © 1987, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1243 印刷版ISSN 0001-8724 医学書院

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