Detection sensitivity of herpes simplex virus DNA in tear fluid by PCR method Hisano Soma 1 , Koji Nishida 2 , Wakako Adachi 2 , Shigeru Kinoshita 2 1Dept of Ophthalmol, Uji Tokushukai Hosp 2Dept of Ophthalmol, Kyoto Prefectual Univ of Med pp.603-606
Published Date 1995/4/15
DOI https://doi.org/10.11477/mf.1410904247
  • Abstract
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We evaluated the sensitivity of polymerase chain reaction, PCR, in detecting herpes simplex virus (HSV)-DNA in graded diluted culture solutions. The sensitivity was 10PFU/ml, independent of whether the tear fluid was obtained with or without Schirmer strips. We applied the method to 6 eyes of herpetic epithelial keratitis or conjunctivitis and to 10 normal eyes. HSV-DNA was detected in all the 6 herpetic eyes and in none of normal eyes. Additional use of image analyzer enabled quantitation of virus concentration in the tear fluid. The present PCR method, in which the sample is collected with Schirmer strips, is useful in diagnosing viral infections in the ocular surface and in estimating the virus concentration.

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