クラミジア病因診断法の比較

Comparison of diagnostic methods for Chlamydia trachomatis

　急性濾胞性結膜炎患者32例の結膜擦過物80検体について，直接螢光抗体法（MicroTrak^®），酵素抗体系（IDEIA^®），DNAプローブ法（PACE^®2）の3種類の非培養検査法とクラミジア分離培養を行った。いずれかが陽性であった42検体中のそれぞれの陽性率は67％，62％，71％および95％であった。症例によっては再燃がみられ，非培養検査法は繰り返して行うことが必要と考えられた。

　We tested 80 conjunctival scraping samples from 32 patients with acute follicular conjunctivitis. We used 4 methods to detect Chlamydia trachomatis: culture, direct immunofluorescent test with (MicroTrak^TM), enzyme-linked immunoassay (IDEIA^TM), and DNA hybridization probe (PACE^TM2). In 42 samples which were positive in one or more methods, the positive rate for each method were 95%, 67%, 62% and 71% in the above order. The non-culture methods became negative in two adults after topical treatment but became positive after several days. When samples are tested by non-culture methods, they should be examined repeatedly for some period.