脳侵襲におけるFAD （Flavin adenine dinucleotide）の影響に関する基礎的研究並びに臨床的研究

Basic and Clinical Studies Concerning the Influences of Flavin Adenine Dinucleotide in Cerebral Injuries

I．緒論

　ビタミンB₂即ちRiboflavinが生体内の物質代謝を円滑ならしめるのに不可欠の物質なる事は今更言を要しないが，1932年Warburg, Chris-tianが黄色酵素と呼ばれる蛋白酵素を研究中，其の補酵素（活性基）がB₂の1—燐酸エステルである事を発見し，B₂が生体内でビタミンとして作用を営むには，其の儘の型では役立たず，其の燐酸エステル型であるFlavin mono-nucleotide（FMN）及びこれにアデニール酸の結合したFlavin adenine dinucleotide （以下FAD）となつて初めて各種酵素系の補酵素として作用し，生体内酸化過程に重要な役割を演ずる事が明らかとなつた。就中FADはFMNよりも更に多くの場合に補酵素となつて居り，実際に脳組織内においてもFlavoproteinの大部分がこのFADの型で存在している。

Effects of FAD nsed in the case of cerebral injury were examined in regard to the respi-ration of brain homogenate.

Hibernation drugs of chlorpromazine and promethazine administered at the concentra-tion of 6×10^-5~6×10^-4 checked the respirati-on of the homogenates. The subsequent use of FAD, it was recongnized, did not work to the effect of checking the respiration any more.

Pharmacologic hibernation with the intrave-nous injection of chlorpromazine checked the respiration to a small extent, the subsequent administration of FAD at the earliest possible moment effected a remarkable increase in the respiration.

A brain homogenate prepared 3 hours after cerebral injury showed a notably decreased respiration, which was well improved by an eary use of FAD.

Also, treatment with pharmacolgic hiberna-tion prevented remarkably a check in respi-ration due to a cerebral injury and maintained the respiration of homogenate at an almost normal level. It was observed that the be-tter respiration of brain tissues was maintai-ned by both treatment of pharmacologic hib-ernation at the time of cerebral injury and the subsequent use of FAD.