実験的脱髄過程の生化学的知見（第1報）

Biochemical Research of Experimental Demyelination (1st Report)

　最近多発性硬化症を初めとして，視束脊髄炎，狂犬病予防接種後脳脊髄炎，麻疹後脳脊髄炎等の脱髄疾患群が本邦の神経^{1）2）3）4）5）}学上にも問題となり，我国に於ける多発性硬化症の存否とか，或いは脱髄機転の成因等が各方面から論議されているにも拘らず，未だ其れ等に関しては不明の点が多く残されている現状である。

　さて髄鞘形成とか脱髄現象と云うのは，組織学的概念に基くものであつて，其の生化学的研究も当然此の概念に基かねばならないのである。

We have studied the biochemical mechanism of experimental brain allergy that is considered one way of demyelination processes. We used Freund's Adjuvant methode to follow the chan-ge of cerebroside and lipoid-phosphorus in the central nervous system and blood of sensitized guinea pigs. The antigen used is the emulsion of adult dog's brain. Cerebroside, one of the protagon, is contained mainly in myelin sheath and it concerns closely with meylination and phylogenetic development.

Cerebroside in the central nervous system of guinea pigs, especially in cerebrum, decreased as the brain allergy or demyelination process advanced, but the blood cerebroside showed tendency of increasing during the course. Li-poid-phosphorus in the Central nervous system and blood of guinea pigs indicated no significant change after antigen injection.

Also to prove the decrease of cerebroside in the central nervous system more accurately, we measured reducing value of sugar separately, which were liberated from gangliosid and sul-phatid by acid hydrolysis. The reducing value of sugar obtained by acid hydrolysis from gan-gliosid fraction indicated no noticeable changes, but that from sulphatid fraction showed slight increasing tendency after antigen injection. We support the view that the decrease of cerebro-side in the brain may be due to distruction of cerebroside by cerebrosidase rather than inhibiti-on of cerebroside synthesis.