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Japanese

A future technique-Non invasive optical methods for the monitoring of cerebral oxygen metabolism by infrared transmission spectrophotometry Masahide Tamura 1 , Hidemitsu Tachibana 1 , Syouji Takeuchi 1 , Yoshihiko Kubo 1 , Natsuki Samejima 1 , Mamoru Tamura 2 , Yasuki Yamazaki 3 11st Dept. Surgery, Asahikawa Medical College 2Research Institute of Applied Electricity, Hokkaido University 3Asahikawa Medical Plant K. K. pp.527-534
Published Date 1987/5/15
DOI https://doi.org/10.11477/mf.1404205059
  • Abstract
  • Look Inside

Biological tissues are relatively transparent to light in near infrared region. Brain oxygen meta-bolism was non-invasively studied by the simulta-neous measurement of hemoglobin (Hb) oxygenation and cytochrome oxidase (Cyt. Aa3) redox state in the rat brain using near infrared transmission spectro-photometer. The spectrophotometers used here were computer-controlled wavelengh scanning and 2-chan-nel dual wavelengh photometers operated with photon counting mode.

Wavelength pairs at 700-815 nm and 815-830 nm were used for the measurements of Hb oxygenation level and of the redox state of cyt. oxidase in situ. Change in the brain blood volume was also monitored by the absorption change at 815 nm.

With anesthetized and artificially ventilated rats, approximately 90% of Hb in the brain was in the oxygenated state at 95% O2+5% CO2 in the inspired gas, which was well coincident with the oxygen saturation of the jugular vein blood. At 20% O2 in theinspired gas, about 70% of the brain Hb was still oxygenated.

Sharp reduction of the oxidase occured at the oxygen concentration below 15% in the inspired gas. But above 30% O2, about 85% of the Cyt. oxidase was in the oxidized state which was almost constant up to 100% O2.

Arteriovenous O2 difference in the rat brain was constant at more than 20% O2 (with 5% CO2), but increased sharply between 10% and 5% 02 in in-spired gas, indicating the oxygen insufficieney in the brain.

Temporally clamping of carotid artery caused deoxygenation of the brain lib, reduction of Cyt. oxidase and a decrease in the blood volume, but completely recovered to the initial levels on the release of clamping.

At 30% O2 respiration, nicardipine (Ca-Antagonist) infusion caused a dose-dependent increase in the brain Hb oxygenation which suggested that nicardi-pine improved the oxygen supply to the brain.

In conclusion, the simultaneous measurements of the Hb oxygenation and mitochondrial redox state can present the further informations about the oxygen balance and various vasodilating actions in the brain in situ.


Copyright © 1987, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1200 印刷版ISSN 0452-3458 医学書院

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