Studies on metabolism of the various parts of guinea pig brain Toyozo Aizawa 1 , Yūichiro Gotō 1 , Fumio Gotō 1 , Fumio Muramatsu 1 , Takashi Ochi 1 , Takamichi Ajiki 1 , Katsuhiko Hamaguchi 1 , Reiko Shioiri 1 , Kichinosuke Sasaki 1 1The department of internal medicine, School of medicine, Keio University pp.586-590
Published Date 1960/4/1
DOI https://doi.org/10.11477/mf.1431906382
  • Abstract
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 Studies were made on the energetic metabolism. of the various parts of guinea pig brain whichcontained cerebral cortex, cerebellar cortex, diencephalon, corpora quadrigemina and the basis ofthe fourth ventricle. Oxygen uptake, lactic acidformation and glucose consumption were examinedrespectively by Warburg's manometrics, Barker-Summerson's method and Somagyis method. Theresults were as follows:

 1. Little difference of oxygen uptake amongthe various parts of the brain was observed. Thehigher lactic acid formation was observed in thebasis of the fourth ventricle and cerebellar cortex, while in cerebral cortex and diencephalon it waslow.

 2. "Potassium-effect" which had been reportedonly in the cerebral cortex slice was also observedin the different parts of the brain. Potassium-effect in the basis of the fourth ventricle was lowerthan that in cortex.

 The metabolism of the slice, which was isolatedfrom the cerebral cortex of the animal inducedto insulin coma, was remarkably decreased, whilethe slice of the basis of the fourth ventricle showedlittle decrease of metabolism in this condition. The number of neuronal and glial cells in thevarious parts of the brain was examined and littlerelation was found between the number of cellsand those reactivity, which was the degree of Potassium-effect or metabolic change in insulin. coma.

 3. a) The grade of oxygen uptake of thevarious parts of the brain in Krebs-Ringer phosphate with various substrates such as glucose,lactate, pyruvate and TCA intermediates showeddifferent results.

 b) The inhibitory action of malonate on Potassium effect was observed in not only cerebralcortex but also the other parts of the brain.

 The relation between energetic metabolism especially the reactivity as described above in the. various parts of the brain and the order of phylogenetical development was considered to beclose, and in every parts of the brain the energeticmetabolism were considered to be carried Out by-way of glycolysis and TCA cycle.

 The differences of metabolic level or reactivity. in the various parts of the brain were consideredto be depend upon the quantitative differencesof the enzyme activity.

Copyright © 1960, Igaku-Shoin Ltd. All rights reserved.


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