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Local gene-transfer into mammalian brain. Masaaki TSUDA 1 1Department of Microbiology, Faculty of Pharmaceutical Sciences pp.35-43
Published Date 1991/2/10
DOI https://doi.org/10.11477/mf.1431900108
  • Abstract
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During the development of the mammalian central nervous sysem (CNS), a large number of brain-specific genes are expressed in a developmentally regulated fashion in brain, a process which is thought to be closely related to the formation of the CNS and the expression of nervous functions. To elucidate this process in relation to the expression and action of brain-specific genes in brain cells, it is desirable to develop a method for introducing foreign genes into the specified areas of brain of living animals. For this purpose, production of transgenic mice is thought to be mostly applicable, but efforts to obtain appropriate control elements to express the foreign genes in a specified area of brain would be needed. Instead of production of transgenic mice, two methods, infection of recombinant viruses and transplanta-tion of transformed cells, are expected to be applicable for introducing foreign genes into specified areas of brain. Infection of recombinant viruses to the brain cells is now using for marking cell lineages of brain cells in vivo, but does not seem to be suitable for expressing foreign genes effectively in a restricted area of brain. Transplantation of transformed cells into brain is suitable for expressing genes coding secretory peptides like growth factors in a restricted area of brain. So far, established cell lines have been used as donor cells, which might cause abnormal proliferation in brain after transplantation. Recently, we have succeeded in transplanting cultured mouse cerebellar cells infected with recombinant retroviruses into the cerebellum of adult mice and expressing introduced gene-products in the host cerebellum for at least 3 weeks after transplantation. However, these methods require laborious experi-mental processes to prepare recombinant viruses or transformed cells prior to transferring foreign genes into brain.


Copyright © 1991, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1243 印刷版ISSN 0001-8724 医学書院

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