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Brain and Nerve No to Shinkei Volume 39, Issue 7 （July 1987）

Brain and Nerve 脳と神経 39巻7号（1987年7月発行）

Japanese English

原著

グリオーマに対するβ-インターフェロンならびにγ-インターフェロンの抑制効果—基礎実験における検討 ANTINEOPLASTIC EFFECT OF β-IFN AND γ-IFN ON MALIGNANT GLIOMAS 中村治 ¹ , 野村和弘 ² , 丸尾幸嗣 ³ , 上山義人 ³ , 松谷雅生 ¹ , 高倉公朋 ⁴ Osamu Nakamura ¹ , Kazuhiro Nomura ² , Koji Maruo ³ , Yoshito Ueyama ³ , Masao Matsutani ¹ , Kintomo Takakura ⁴ ¹都立駒込病院脳神経外科 ²国立がんセンター脳神経外科 ³実験動物中央研究所 ⁴東京大学 ¹Department of Neurosurgery, Tokyo Metropolitan Komagome Hospital ²Department of Neurosurgery, National Cancer Center ³Central Institute of Experimental Animals ⁴Department of Neurosurgery, University of Tokyo Hospital pp.627-632

発行日 1987年7月1日 Published Date 1987/7/1

DOI https://doi.org/10.11477/mf.1406205933

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Abstract 文献概要
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　抄録　β-interferon （IFN）ならびにγ—IFNの抗腫瘍効果をin vitro, flow cytometry，ヌードマウスモデルを用いて検討した。in vitroではβ-IFNは濃度依存性に腫瘍抑制効果を示すのに対し，γ-IFNではほとんど効果がみられなかった。一方DNAヒストグラムに及ぼす影響については，β-IFNではS期，G2M期への集積傾向が強いのに対し，γ-IFNでは，ヒストグラム上の変化はほとんど認められなかった。ヒトglioma移植ヌードマウスにおける検討では，β-IFN投与群での治療群／対照群体積比（T／C）が44％であるのに対し，γ-IFN投与群のそれは80％であり，γ-IFNの抑制率は低かった。β-IFNについては，既に報告したように，glioma 6株中2株に有意な腫瘍抑制効果を認め，本glioma株においても効果がみられた。一方γ-IFNの本glioma株に対する効果—少くとも直接効果は乏しいと考えられるが，γ-IFNについては，免疫防御機構を介した作用が強いと言われており，その薬力学的作用についての解明が肝要である。

We have compared the anti-tumor effects of β-and γ-interferons (IFN) against human glioma cells using in vitro model, flow cytometry and xenograft in nude mouse. Contrary to γ-IFN which had a negligible effect on the growth of glioma cells, β-IFN showed a dose dependent growth-inhibitory effect on glioma cells in vitro. Our flow cytometry studies revealed that proportion of DNA contents which represent the S and/or G2M phase of cell cycle was markedly increased in cells treated with β-IFN. However, in cells treat-ed with γ-IFN, no pertubations in cell cycledistribution were observed. Regarding the anti-tumor effect examined using xenograft of human glioma cells in nude mouse, the tumor reduction rate which was calculated from the ratio of tumor volume between treated (T) and control (C) groups and expressed as T/C value, was 0.44 in animals treated with ,β-IFN. However, T/C value in animals treated with γ-IFN was 0.80 which was derived from the weaker anti-tumor effect of γ-IFN as compared with that of β-IFN. Beta-IFN was found to cause a partial suppression of the growth of glioma strain used in this studywhich confirms our previous report that β-IFN is effective in reducing tumor size in 2 of 6 glioma strains tested. On the other hand the anti-tumor effect of r-IFN, at least in terms of direct effect, against glioma cells used in this study was minimum. However, the mechanism of γ-IFN's anti-tumor effect may exert mainly through the potentiation of immuno-defence me-chanism and the further studies on its pharmaco-dynamics will be necessary to evaluate the anti-tumor effect of γ-IFN.