脳代謝サイクルの中間代謝物質による頭部外傷の治療—第2報 CDP-Cholineの代謝に関する基礎的研究

TREATMENT ON HEAD INJURIES WITH INTERMEDIATE SUBSTANCES OF METABOLIC CYCLE OF BRAIN:II. Basic Study on Metabolism of Cytidine Diphosphate Choline

I．緒言

　先に三宅^1）らは，頭部外傷による意識障害の治療に対してCDP-cholineを用いた症例について検討し，この薬剤が臨床経過を短縮好転させる可能性を持つことを報告した。

　その後われわれはCDP-cholineの作用機序を解明すべく主としてその代謝の面から実験的検討を行なつた。

The authors studied about the metabolism of CDP-Choline in brain tissue in vitro and in vivo using FP-CDP-Choline by radioautography.

CDP-Choline in medium was taken up into cultured nerve cells.

CDP-Choline was incorporated into nucleus within 2 hours but succeedingly spread to cytoplasm and nerve processes.

The uptake of CDP-Choline into the cells was en-hanced by simultaneous administration of ATP.

The same experiment was carried out on glia cells and ependymal cells, but they did not incorporate CDP-Choline so much as nerve cells did even if ATP was administrated.

The authors administrated I-P-CDP-Choline in-traperitoneously to the rat which had been induced cold injury with aceton-dry ice over the scalp, and confirmed that grains of H³⁾-CDP-Choline was noticed mainly in white matter especially in the location of the injuries. The incorporation was also enhanced by administration of ATP. We may be able to advise that in clinical cases it is more prefarable to apply CDP-Choline with ATP.

And these facts lead us following hypothesis, namely lipid metabolism of cerebral white matter is enhanced in cases of head injuries and followingly CDP-Choline, which is supposed to he required to lipid metabolism, is much taken up into them. It may be utilized to protect further injuries or damages of myelin struc-ture.