網膜芽細胞腫のレクチン結合部の組織細胞化学的検討

Lectin binding pattern in retinoblastoma

　特定の糖構造と特異的に結合する糖結合蛋白であるレクチンを用いて，網膜芽細胞腫におけるレクチン結合パターンを，光顕ならびに電顕下で，組織細胞化学的に検索し，正常家兎網膜およびヒト網膜の場合と比較検討した．

　正常家兎網膜では，peanut agglutinin （PNA）は杆錐体層，外顆粒層，Ricinus communisagglutinin （RCA）は外顆粒層．内境界膜，神経線維層，wheat germ agglutinin （WGA）は杆錐体層，concanavalin A （ConA）は全層にわたってそれぞれ反応陽性であった．

　正常ヒト網膜では，WGAは杆錐体層に強陽性，ConAは全層にわたり陽性反応を呈した．

　網膜芽細胞腫では分化型，未分化型共にWGAとConAとが陽性であった．ロゼット形成細胞では，組胞膜に反応陽性であり，特にロゼット中心に一致して強陽性であった．

　以上の結果から，特にWGAを用いた場合．網膜芽細胞腫と視細胞に共通の糖鎖構造が存在することが示唆された．

We studied the binding of seven different kinds of lectins to the retinoblastoma of 4 eyes and com-pared with that to the normal human and rabbit retinas. Peroxidase labelling method was used forlight and electron microscopic observation of lectin binding pattern.

In the normal rabbit retina, wheat germ agglu-tinin (WGA) stained the rod and cone layer and the outer nuclear layer. Peanut agglutinin (PNA) stained the rod and cone layer and the outer nuclear layer. Ricinus communis agglutinin (RCA) stained the outer nuclear layer, the inner limiting layer, and the nerve fiber layer. Concanavalin A (ConA) stained all the layers of the retina.

In the human retina, WGA stained the rod and cone layer, whereas ConA stained all of the layers.

In the human retinoblastoma, ConA stained the cytoplasm and the cell membrance of both the differentiated and the undifferentiated types. By electron microscopy, the cell membrane, the nuclear envelope, and the endoplasmic reticulum were stained. WGA stained the cell membrane of the cells of both types. A particularly strong staining was observed in the central part of the Flexner-Wintersteiner rosette formation. These results sug-gest that the retinoblastoma is similar in structure to the photoreceptor cells in terms of the saccharide components.