Japanese
English
- 有料閲覧
- Abstract 文献概要
- 1ページ目 Look Inside
成熟砂鼠の海馬における糖鎖構造の分布を,7種類のレクチン,即ちconcanavalin A(Con A),wheat germ agglutinin(WGA),Ricinus communis agglutinin(RCA),Phaseolus vulgaris agglutinin(PHA-E),peanut agglutinin(PNA),soybean agglutinin(SBA),およびVicia villosa agglutinin(VVA)を用いて組織化学的方法により検索した。Con AおよびPHA-Eでは核膜がそれぞれ強度陽性ないし中等度陽性を示したのに対し,WGAでは細胞膜のみが陽性であった。血管内皮細胞はRCAに対して強陽性となり,内皮細胞同定のマーカーとしてのRCAの有用性が示唆された。アンモン角の一部の神経細胞とCA 2およびsubiculumに接するCA 1領域の細胞体周囲組織がSBA,VVAに対して特異的に陽性となったが,歯状回では陽性細胞が認められなかった。一方PNA反応では,アンモン角および歯状回の一部の神経細胞の細胞膜が陽性となった。これらの結果より,海馬内神経細胞に含まれる糖鎖構造は細胞により異なることが指摘され,細胞形態による分類,神経伝達物質による分類とともに,糖鎖構造の相違が神経細胞を分類する場合の有用な手段となると考えられる。
Paraffin-embedded sections of gerbil hippocampus were made and stained by use of lectins with different sugar specificities : Concanavalin A (Con A) , wheat germ agglutinin (WGA) , Ricinus communis agglutinin (RCA), Phaseolus vulgaris agglutinin (PHA-E), peanut agglutinin (PNA), soybean agglutinin (SBA), and Vicia villosa agglutinin (VVA) to investigate the distribution of glycoconjugates in this brain region.
Nuclear membranes of all the neurons in the hippocampus were positively stained with Con A and PHA-E, whereas WGA revealed definite staining of cell membranes. Endothelial cells of blood vessels were intensely positive to RCA, suggesting its usefulness as a marker of endothelial cells. With SBA and VVA, a few neurons in Ammon's horn were positively stained, while no positive cells were observed in the dentate gyrus. CA2 and the medial part of the CA1 were also positive bilaterally with SBA and VVA. Some neurons in Ammon's horn and dentate gyrus were selectively stained on the surface of their cell bodies with PNA.
The present results show that lectins used distinguish different subpopulations of hippocampal neurons, in-dicating a new possible classification of hippocampal neurons based on their differences in glycoconjugates.
Copyright © 1991, Igaku-Shoin Ltd. All rights reserved.