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METHOD OF CULTIVATION OF CHLAMYDIA TRACHOMATIS Taiji Nishimura 1 , Hiroyuki Abe 1 , Kazuhiro Yoshida 1 , Masao Akimoto 1 , Toshimitsu Konjiki 1 , Hiroshi Kawai 1 1Department of Urology, Nippon Medical School pp.771-774
Published Date 1981/8/20
DOI https://doi.org/10.11477/mf.1413203192
  • Abstract
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Method of cultivation of Chlamydia trachomatis from urethral discharge of men with gonococcal and non-gonococcal urethritis was briefly described as a preliminary report.

Cultivation was performed on McCoy cells with or without pretreatment by antimetabolites after centrifugation at 33℃ at 4000g for 1 hour.

As a result, satisfactory isolation rate was obtained regardless of pretreatment by antimetabolites.

C. trachomatis was isolated from 3 of 11 patients with non-gonococcal urethritis and 1 of 14 patients with gonococcal urethritis.


Copyright © 1981, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1332 印刷版ISSN 0385-2393 医学書院

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