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Virological analysis of intraocular fluids over time in acute retinal necrosis Yasuyuki Yamauchi 1 , Hiroshi Minoda 1 , Mitsutoshi Kasiwase 1 , Norio Usui 1 , Katsutoshi Yokoi 1 , Toshihiro Ichikawa 1 , Jun-ichi Sakai 1 , Masahiko Usui 1 1Department of Ophthalmology, Tokyo Medical College Hospital pp.603-606
Published Date 1997/4/15
DOI https://doi.org/10.11477/mf.1410905317
  • Abstract
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We tested intraocular fluids in 23 cases of acute retinal necrosis regarding viral antibody titer and polymerase chain reaction (PCR). Aqueous humor was obtained in 22 samples and vitreous aspirate in 17. Aqueous sample obtained during the early phase of the disease showed positive PCR identification of the causative virus but unremarkable viral antibody ratio. Vitreous samples showed similar results in the incidence of positive PCR results and viral antibody ratio regardless the timing of sampling. As an overall finding, samples taken before 20 days after onset of disease showed higher rates of positive PCR findings. Samples taken thereafter showed higher incidence of viral antibody titer. The findings show that PCR studies are indicated for the early stage of the disease and that viral antibody studies are more sensitive for later stages.


Copyright © 1997, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1308 印刷版ISSN 0370-5579 医学書院

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