IL−2活性化リンパ球の悪性脳腫瘍への浸潤能—養子免疫療法の基礎的研究

The Motility of IL-2 Activated Lymphocytes into Malignant Glioma

　悪性神経膠腫培養上清（GCS）とinterleukin−2（IL−2）がASL（autologous stimulated lymphocytes）の運動能に及ぼす影響について，collagen gel, chemotaxic chamber, flow cytometryを用いて分析した。GCSはASLのcollagen gel内への浸潤を抑制したが，運動能自体は抑制せず逆に増強した。また，Tリンパ球の活性化・接着能を反映するCD 26抗原を発現し維持した。他方IL−2はASLのcolagen gel内への浸潤を抑制した。運動能に対しては明らかな影響は与えなかったが，CD 26抗原を高率に発現し維持した。この実験結果と臨床組織標本の所見から，ASLを用いた局所養子免疫療法を効果的に行うためには，（1）腫瘍摘出腔内に過剰のIL−2を投与しないこと，（2）1回当りのリンパ球数を多くして注入回数を減らすこと，（3）リンパ球注入前に腫瘍をできるだけ摘出してその内腔を洗浄することが重要である，と結論された。

The effects of glioma culture supernatant (GCS) and interleukin-2(IL-2) on the motility of autologous stimulated lymphocytes (ASL) were studied by using collagen gel system, chemotaxic chamber system and flow cytometric analysis. GCS inhibited the migration of ASL into collagen gel. It enhanced the ASL motility and the expression of CD 26 antigen on the cell surface. IL-2 inhibited the migration of ASL into collagen gel and had no influence on the motility of ASL, but enhan-ced the expression of CD 26 antigen. On the other hand, a clinical glioma specimen showed limited depth of ASL migration when injected into the tumor cavity in addi-tion to the formation of fibrin like membrane on its surface and a layer of degenerated ASL under it.

To make ASL therapy more effective to malignant glioma, the following measures should be recommend-ed ; 1) inject adequate volume of IL-2 into tumor cav-ity, 2) reduce the frequency of ASL administration, 3) wash out of glioma secreting factors, degenerated ASL and glioma cells in addition to reduce the volume of tumor tissue.