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IMMUNOLOGICAL SURVEILLANCE SYSTEM OF A RAT BRAIN TUMOR MODEL : 1. ACCELERATION OF TUMOR TAKE BY A NEW METHOD OF TRANSPLANTATION (SOFT AGAR TECHNIQUE) Hachiki Sobue 1 1Department of Neurosurgery, Brain Research Institute, Niigata University pp.533-538
Published Date 1980/5/1
DOI https://doi.org/10.11477/mf.1406204588
  • Abstract
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Intracerebrally inoculated tumor cell suspention was liable to overflow from the cerebral parenchyma because of its counter pressure. And we originally thought that it was a major cause for the low incidence of tumor take and the unevenness of tumor growth. Then we designed a new method of"Soft Agar Technique"for the aculate trans-plantation of RG-C6 glioma cells into the brain of allogeneic WKA rats, and prepared a brain tumor model.

Centrifugated tumor cell pack was mixed quickly and uniformly with agarose sol kept at 37℃. The TRYPTO-SOY AGAR, commonly used for the culture and separation of bacterias, was better for the maintenance of viability of the tumor cells. This agarose sol easily changed into gel in room temperature and we inoculated the gel with tumor cells into the brain. We made it a role to inocu-late 3 ul of volume for the prevention of brain damage. The optimum concentration of agarose for the maintenance of sol at 37℃ was 1.0%. The optimum number of tumor cells to get a uniform mixture in the agarose sol, was 1×106/3 ul. And in this condition we could get 89.7% of incidence. Inoculating 1×106/3 ul of tumor cells in the usual form of cell suspention, the incidence was only 35.5%.

We killed the rats at random days post-implant, and analysed the tumor growth. The growth index was calculated by weighing and comparing the percentage of tumor area to the figure of the entire coronal section. Generally the tumor growth curve was represented in a straight regression line,with statistical significance. Investigating the tumor growth in the period from the 20th to the 30th day after transplantation, the gradient of the growth curve tended to become steep. The brain tumor had a diameter of about 8 milimeters on and after the 30th day post-implant.

Subcutaneously inoculating 1×106 of tumor cells in the form of cell suspension, they were rejected promptly and there was no tumor growth. In order to get 100% of tumor take, it was necessary to inoculate 10 times that number into the brain. The growth index was taken by multiplying the major to minor axis of the tumor. The tumors increased in size in about 8 days post-implant, and then regressed. They dissapeared on the 17th day post-implant. The subcutaneous tumor, at the stage of maximum growth, had a diameter of more than 8 milimeters.

As compared with the subcutaneous tumor growth, brain tumors grew more slowly. And gathering from the cell cycle time of RG-C6 IN VITRO, we could not attribute this finding to the difference of innoculated tumor cell numbers be-tween the both. In order to get a solution to this problem, we cultured the agarose droplets with 1×106 of tumor cells in TC-199 medium. Then, tumor cells began to spread out from the surface of droplets in the process of time and increased in number up to 48 hours after cultivation. However most cells in the droplets remained out of the tumor growth.

If this condition might happen also IN VIVO, it was surmized that the small numbers of tumor cells rather originated the high incidence of tumor take, contrary to our original expectation. Refer-ring to this attractive results, we must give our mind to the existance of "Dilution Escape Phenomenon"in the brain. And in this condition, intracerebrally inoculated tumor cell growth may well have been retarded.


Copyright © 1980, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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