フオルマリン固定、パラフイン包埋の同一切片による中樞神經系構成分の特別染色（豫報）

STAINING PROCEDURE FOR PARAFFIN OF FORMALIN FIXED CENTRAL NERVOUS TISSUE.(PRELIMINARY REPORT)

緒言

　吾々が，中枢神經系の構成分について，特別染色をなさんとする場合，その染色手技の煩雑なことも勿論ではあるが，それにも増して，固定，切片作製の區々なる點に，少なからぬ困難を感ずる。

　かゝる見地より吾々は，最も普遍的なフオルマリン固定によるパラフイン切片について，これらの特別染色が果して可能なるものか否かを見極めたいものと考え，表題に示すが如き研究を思い立つたのである。

Proceding of formalin fixing paraffin sectioned material to perchase for the various const-ructionof central nervous system. I tried a certain particular stain, and was able to get some considerable results. So I report it on this paper.

(I) Staining for axis-cylinder.

a) Silver impregnation for axis-cylinder.

1) Remove paraffin from section and place in;

2) 5% ammoniac iodine potassium solution, half to an hour at 55-60 C grade.

3) 30% formalin for 1 minute.

4) 10% silver nitric acid, 15 minutes at 55-60 C grade.

5) and deoxydation in 20% formalin.

6) 3 times diluted Gros-Schultze's ammo niac silver fluid, 15 minutes at 55-60 C grade.

7) Deoxydation in 20% formalin.

8) And follow the precedent of Bielscho-wsky's method. (Gold chloride etc.) b) Hematoxylin staining for axis-cylinder. This procedure on cut sections of the cerebellum, pons, and of medulla oblon-gata is described, allowing Sargent's he-matoxylin, is worked excellent consequ-ence.

(Ⅱ) Staining for medullary sheath.

I deviced four methods, however, one of what altered of Kulschitzky's method was most available for this purpose.

1) Remove paraffin by xylol.

2) 5% Iron alum, 15 minuites.

3) Kulschitzy's hematoxylin, 20 to 30 minu-tes at 55-60 C grade.

4) Differentiate in lithion-ferricyan potas-sium.

5) Dehydrate and mount.

(III) For Nissl staining

This method should be proceded with original solution of Wrights' was deluted 3 times by Giordano buffer solution, and be differentiated by absolute alcohol.

(IV) Staining for glia fibrils.

a) The manner specialized in using of Mallo-ry's hematoxylin.

1) Of high grade acidfied acetic acid copper solution, an hour to one and half hours at 37 C grade.

2) 0.3% permanganic acid potassium, 30 minuites to an hour.

3) 1.0% oxalic acid, 30 mminuites and place in;

4) Mallory's hematoxylin, 10 hours or over at 37℃ grade.

5) Dehydrate and mount.

b) Method in using of victoriablue. 1) Excessively acidfied saturation acetic acid copper solution, one and half to two hours at 37 C grade.

2) 0.3% permanganic acid potassium, an hour or over.

3) 1.0% oxalic aJid, 30 minuites.

4) 1.0% victoaiablue, 4 to 5 hours or over at 37℃ grade.

5) After the manner of the Weifert's me-thod. (Iodine-iodic potassium solution and anilin-xylol. etc.)