フォルマリン固定，パラフィン包埋の同一切片による中樞神經系構成分の特別染色（第2報竝總括）

STAINING PROCEDURE FOR PARAFFIN OF FORMALIN FIXED CENTRAL NFRVOUS TISSUE. (II. REPORT WITH THE SUMMERIZASION.)

　われわれは表題の研究について，その前半を第八回日本腦神經外科學會に，その要旨を本誌（第四卷四號）に發表した。その後本法の改良と新法の發見に努力し，二三の見るべきものがあつたので報告する。

　一般染色はともかく，特殊染色の場合には一度の染色で常に必ず好結果を得るとは限らないが，「容易な手技と，多くの利點」というわれわれの目的に對して，繊細な線維の表現を必要とする軸索，神經膠線維の染色や，固定液によつて甚だ不定なNissl氏顆粒染色なども，パラフイン切片法によつて概ねその目的を達することができると思う。

　Concerning with our preliminary reports which we mentioned previously, there were somewhat unsatisfied points, so we report the additional results, with some new methods.

　A) For axis-cylinder.

Silver impregnation which was described in preliminary report, is so unfavorable of its stainablity in summer, that we suggest some improving ways including the following points;

　Shortening of the duration in water tapping should be considered (usage of the water supply),

　Repeating (twice) of ammoniac silver impregnation (seems good for it).

For the hematoxylin staining, the section may be oxyfled by acidual saturation acetic copper solution (sat. acetic copper sol. 97cc. glacial acetic acid 3cc. reagent quality) and then stain by hematoxylin after treating of saturation acetic acid copper sol. and differentiate with borax-ferricyan potassium (Weigert).

　B) For staining of medullary sheath, especially we prove to recommend to the Heindenhain and Kulsehitzky's method as we described also in preliminary report.

　C) For Nissl Gaining.

On using of Mc Ilvaine's buffer solution, the cellular architectural components of the ectodermal derivatives, are presumably able to obtain the best results proceduring as follows;

　　1) Remove paraffin by xylol.

　　2) Place in thionin solution (1% thionin solution 100cc. glacial acetic acid 1 gtt.) for 10 minues.

　　3) Differentiate in anilin-alcohol (1: 9).

　　4) Xylol, balsam.

　D) For neuroglial fibrils.

Both victoria blue and Mallory's method which were mentioned in preliminary report, are altered in details, and added to that, a new method of methyl violet staining is described by us.

　a) The manner specialized in using of Mallory's hematoxylin.

　　1) 0.3% permanganic acid potassium, 22 minutes.

　　2) 1.0% oxalic acid, 20 minutes.

　　3) Mallory's phosphotungstic acid hematoxylin, 10 hours or over, at 37-38℃ grade,

　　4) then place in incubator (55-60′C) for half to an hour,

　　5) and keep it an hour or over in room temperature.

　　6) dehydrate and mount.

　b) Method in using of victoria blue.

　　1) Acidual saturation acetic copper solution, an hour or over at 55-60′C grade.

　　2) 0.3% permanganic acid potassium, 30 minutes.

　　3) 1.0% oxalic acid, 30 minutes.

　　4) 1.5% victoria blue, 4 to 6 hours at 37′C grade.

　　5) After the manner of the Weigert's method.

　c) Method in using of methyl violet.

　　1) 0.3% permanganic acid potassium, 30 minutes.

　　2) 1.0% oxalic acid, 30 minutes.

　　3) Saturation phosphomolybdenic acid alcohol solution (1: 4), 10 minutes.

　　4) Methyl violet solution (1% methyl violet 5B sol. (Grübler) 35.0cc. abs. alcohol 15.0cc. carbolic acid solution 2.5cc.), 30 minutes.

　　5) After the manner of the Weigert's method.