Modification of Leukocyte Kinetics in Normal Pulmonary Microcirculation Caused by Adhesion Molecules Nagato Sato 1 , Yukio Suzuki 2 , Kazumi Nishio 1 , Takuya Aoki 1 , Koichi Suzuki 1 , Katsuhiko Naoki 1 , Hiroyasu Kudo 1 , Kuniharu Tsumura 3 , Kazuhiro Yamaguchi 1 1Department of Medicine, School of Medicine, Keio University 2Department of Internal Medicine, Kitasato Institute Hospital 3Biomedical Department, Sankei Corp. Keyword: 肺微小循環 , 白血球動態 , 接着分子 , pulmonary microcirculation , leukocyte kinetics , adhesion molecule pp.489-494
Published Date 1998/5/15
DOI https://doi.org/10.11477/mf.1404901693
  • Abstract
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In order to elucidate the possible roles of various adhesion molecules in modifying the leukocyte behavior in the pulmonary microcirculation, we investigated. based on the isolated perfused lungs prepared from normal rats, the leukocyte kinetics in pulmonary arter-ioles, venules and capillaries under conditions in which functions of adhesion molecules were appropriately inhibited. Observation of leukocyte behavior and deter-mination of microvascular architecture were precisely made by using a real-time confocal laser luminescence microscope. All the confocal images thus obtained were recorded on a high-speed and high-sensitivity video camera. The significant reduction in leukocyte velocity (i. e. leukocyte rolling) due to interfering interactionsbetween the endothelium and the leukocyte was inves-tigated in both arterioles and venules. However, the relative frequency of leukocytes with rolling was much higher in arterioles than that in venules. Arteriolar rolling of unstimulated leukocytes was mediated via a L selectin dependent mechanism, while venular rolling was governed by an ICAM-1 related mechanism. The entrapment of unstimulated leukocytes in capillaries was not conspicuously influenced by any adhesion mole-cules including ICAM-1 as well as P-and L-selectins. Stimulation of leukocytes with cvtokine - induced neutrophil chemoattractant/gro (CINC/gro) which upregulates CD 11/CD 18 on the leukocyte surface significantly enhanced the numbers of rolling leukocytes in venules and of entrapped leukocytes in capillaries. These experimental results may suggest that leukocyte kinetics in the normal pulmonary microcirculation in-I eluding arterioles, venules and capillaries is modified by different classes of adhesion molecules.

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