脳腫瘍のsquash標本による悪性度評価のためのKi−67 PAP染色

K1-67 PAP STAIN FOR HISTOLOGIC GRADING OF BRAIN TUMORS BY SQUASH PREPARATIONS

　抄録　増殖期にある細胞の核抗原と反応するKi−67モノクローナル抗体によるperoxidase-antiperoxi-dase （PAP）染色を141神経系腫瘍のsquash標木に施行した。同一腫瘍組織からの凍結切片にもKi−67 PAP染色を施行した36例では，両者の陽性率は高い相関を示した。squash標本における陽性率は，これまでの凍結切片による著者らの報告同様，組織学的悪性度と良好な相関を示した。即ち，転移癌の平均陽性率は26.7％，glioblastomaでは12.6％，良性のmesodermalの腫瘍では2.8％以下であった。squash標本によるKi−67陽性率と陽性細胞の形態は，摘出組織の術中迅速組織診断，および悪性度評価に有用であり，経蝶形骨洞手術および定位脳手術により得られた極めて小さな組織に適用できる。

Using Ki-67 monoclonal antibody to the nuclear antigen of proliferating cells, the squash prepa-rations of 141 brain tumors and the 36 frozen sections from corresponding tumor tissues were stained with peroxidase-antiperoxidase method. The staining indices in squash preparations corre-lated well to those in the corresponding frozen sections. There were good correlations between the percentage of stained cells and the histologic grade in agreement with known biologic behavior. In order of decreasing malignancy, the averages of Ki-67 staining indices were 26. 7% for metastatic carcinomas, 12. 6% for glioblastomas, and less than 2. 8% for benign mesodermal tumor groups.

Ki-67 staining with squash preparations could be applied to small tissues obtained by transsphe-noidal surgery and stereotactic surgery as valuable adjuncts to intraoperative histologic diagnosis and the estimation of histologic malignancy.