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Cell adhesion molecules of immunoglobulin superfamily―molecular genetics of neural cell adhesion molecule L1. Masayuki MIURA 1 , Keiichi UYEMURA 1 1Department of Physiology, Keio University School of Medicine pp.797-812
Published Date 1992/10/10
DOI https://doi.org/10.11477/mf.1431900276
  • Abstract
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During the development of nervous system, cell-cell communications are mediated by various molecules named cell adhesion molecules. Among them, a group of cell adhesion molecules found in the nervous system bears strong homologies to immunoglobulins. These molecules are termed the immunoglobulin superfamily. Neural cell adhesion molecule L1 is a 200 KDa glycoprotein which is involved in many critical phenomena such as neuronal migration, adhesion, neurite outgrowth, fasciculation and myelination. Complementary DNA (cDNA) cloning of mouse L1 has revealed that this molecule is a member of the immunoglobulin superfamily. To perform the molecular genetic analysis of L1, we have isolated and sequenced a full-length cDNA encoding the rat and human L1. In both species, the deduced amino acid sequence as a whole shows high homology to mouse sequence. In addition to this complete form of L1, we found an isoform, L1es, which lacks four amino acid residues (RSLE) in the cytoplasmic domain and is derived by tissue-specific alternative splicing. While L1 mRNA was predominantly expressed in the brain, L1cs was found exclusively in peripheral nervous tissue. We transfected rat L1 cDNA into mouse fibroblast L cells. Stable transformants expressing L1 showed uniform cell surface expression of the molecule without phenotypic changes. An L1-expressing transfectant acquired the cell adhesion capacity in a homophilic manner and markedly enhanced neuronal cell adhesion and migration in coculture with rat cerebellar neurons. These results indicate that L1 is involved in many critical periods in neural development through molecular interactions.


Copyright © 1992, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1243 印刷版ISSN 0001-8724 医学書院

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