High throughput genotyping system Ryozo Kuwano 1 1Department of Bioinformatics, Genome Science Branch, Center for Bioresource-based Researches, Brain Research Institute, Niigata University Keyword: 多型解析 , マイクロサテライト , SNP , アルツハイマー病 pp.669-676
Published Date 2004/10/10
DOI https://doi.org/10.11477/mf.1431100222
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 Complex diseases are caused by combination of multiple genetic and environmental factors. To understand the etiology of the disease, identification of these genetic factors is the most important way. We set up the high throughput genotyping system to discover genetic risk factors. We introduced 2D-code for anonymous numbering of samples from many institutes, and stored these 96 samples in one box as an analysis unit. A bar-cord label was pasted on the 96 well and 384 well type plates to trace the reactions, and to identify the sample in a well. Search of the genetic polymorphism marker was performed by data base-assisted bioinformatics analysis of the candidate locus. Genotyping of 8markers and 384 samples(=4 boxes)was carried out at once. The genome database is build from the individual genotyping data using microsatellite or SNP as markers and their allele frequency in Japanese population. The clinical database is construction of diagnosis and treatment of disease, gender, onset age, other clinical examination data and so on under the protection of privacy. The clinical database can be used to link to the genome database and marker database for statistic analysis. By using this system the genetic risk factor are able to identify through candidate gene based approach or genome wide scan. This system can be also used genetic linkage analysis for a family based disease and association study for common disease. Equipments composed the genotyping system are connected in the intra-network frame.

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