Clinical implication of rapid diagnostic tests in adenoviral conjunctivitis Waka Saitoh 1 , Norihiko Itoh 1 , Shigeaki Ohno 1 , Akira Oshima 1,2 , Haruhiko Nakajima 2 , Hiroaki Ishiko 2 , Natsuko Taniguchi 3 , Khoki Aoki 3 1Dept of Ophthalmol, Sch of Med, Yokohama City Univ 2Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., 3Aoki Eye Clinic pp.609-612
Published Date 1996/4/15
DOI https://doi.org/10.11477/mf.1410904826
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We evaluated the value of rapid diagnostic tests in 49 patients suspected of viral conjunctivitis. The tests included culture isolation, enzyme-linked immunosorbent assay (ELISA) and PCR-RFLP which is a combination of polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (RFLP). Each of the three tests was evaluated as to sensitivity and specificity. We defined PCR-positive cases as adenoviral conjunctivitis. Their clinical features were compared with those of nonadenoviral conjunctivitis. Out of the 49 patients, 63% were positive for PCR, 59% were positive for culture isolation, and 33% were positive for ELISA. Incidence of severe conjunctivitis, corneal complications, lymphadenopathies and pseudomembranes were significantly higher in patients with adenoviral conjunctivitis than in nonadenoviral one. There was no false negative case for PCR-RFLP in the present series. PCR-RFLP analysis, therefore, seems to be an accurate method in the diagnosis of adenoviral conjunctivitis.

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