5検査施設間での白内障術前結膜囊培養結果の比較

Bacterial flora in the conjunctival sac prior to cataract surgery cultivated in five laboratories

要約　目的：検査施設が異なることにより，結膜囊常在菌培養結果に違いがあるかを検討する。対象と方法：白内障手術予定の患者500眼を対象とした。術前の結膜囊擦過検体を100検体ずつ5検査施設に細菌培養同定検査依頼し，結果を比較した。結果：細菌検出率は各検査施設で45，74，80，84，89％であり，検出株数はそれぞれ47，80，122，137，186株であった。全検査施設に共通してS. aureus，Enterococcus属，Enterobacter属が同定された。P. acnesは2施設でのみ，S. epidermidisは3施設だけで報告があった。結論：検査施設により結膜囊常在菌の細菌検出率，検出株数や菌種検出傾向に差が認められた。これは培養条件や検査方針が異なることが原因と考えられた。細菌培養検討の際には，検査施設の選定に注意が必要であることが示された。

Abstract.　Purpose：To report the incidence of bacterial flora in the conjunctival sac in asymptomatic eyes cultivated in five laboratories. Cases and Method：This study was made on 500 eyes scheduled for cataract surgery. The series comprised 201 male eyes and 299 female eyes. The age averaged 73 years. Scrapings were obtained from the conjunctival sac prior to surgery. One hundred samples each were sent to five laboratories for culture and identification of bacteria. Results：Incidence of bacterial organisms was 45, 74, 80, 84 and 89％ respectively. Number of strains was 47, 80, 122, 137 and 186 strains respectively. S. aureus, Enterococci and Enterobacter were reported from all the five laboratories. P. acnes was found in 2 laboratories only. S. epidermidis was found in 3 laboratories only. Conclusion：There were differences in the reported rate of detection, type of cultured bacteria, and number of strains from five laboratories. The observed differences may reflect different methods of culture and policy of laboratories. Due attention is necessary in selecting laboratories for bacterial studies.