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Alterations in Cytokinetics and Heat Shock Protein (70kDa) Expression of Glial Cell by Hyperthermia Takahisa Fuse 1 1Department of Neurosurgery, Nagoya City University Medical School Keyword: heat shock protein , glioma , hyperthermia pp.843-850
Published Date 1991/9/1
DOI https://doi.org/10.11477/mf.1406900243
  • Abstract
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Expression of major heat shock and stress-in-duced protein, HSP70, is known to be under com-plex regulation in tumor cells. In this study, we investigated the alternations of cytokinetics and HSP70 expression by hyperthermia in the invitro experimental systems, using two rat glioma cell lines, two human glioblastoma cell lines and rat glioblast cells. For hyperthermal treatment the flasks were placed in water baths warmed up at 41 -45℃ for 15 min. To determine the effect of hyper-thermia on the cell cycle progression, the changes in the DNA distribution of the cell population werestudied by flow cytometry (FCM). The levels of HSP70 protein were determined by immunoblot analysis. The relationship between cell cycle and HSP70 expression was investigated by FCM using PI and FITC-labelled HSP70 double staining tech-nique. These results were as follows : 1) Compared with the control, hyperthermic treatment at 42℃ or 44℃ caused both 354A and T98G cells to accumu-late in S phase 18 hours after treatment and G2/M phase after 6-18 hours. 2) Hyperthermic treatment at 42℃ caused C6 cells to accumulate in S phase 6 hours after treatment, whereas heat treatment at 44℃ caused C6 cells to accumulate in S phase after 18 hours and G2/M phase after 6 hours. 3) A172 cells were accumulated only in G2/M phase by hyperthermia. 4) Glioblast cells did not show the alterations of cytokinetics by heat treatment remarkably. 5) HSP70 protein synthesis were en-hanced under hyperthermic conditions in all type of cells, whether primary glioblast or permanent glioma cell lines. The increase of HSP protein synthesis was particularly enhanced in the cell lines, which cytokinetics remarkably altered due to S phase block and G2/M block by heat treatment. 6) The maximum expression of HSP70 was detected in the cells at G2/M phase, indicating that HSP70 expression would be regulated during the cell cycle.


Copyright © 1991, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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