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Brain and Nerve No to Shinkei Volume 43, Issue 1 （January 1991）

Brain and Nerve 脳と神経 43巻1号（1991年1月発行）

Japanese English

原著

神経膠腫の腫瘍血管の微細構造—血管透過性に関する形態計測的検討 Ultrastructure of Glioma Vessel : Morphometric Study for Vascular Permeability 高野晋吾 ¹ , 吉井与志彦 ¹ , 能勢忠男 ² Shingo Takano ¹ , Yoshihiko Yoshii ¹ , Tadao Nose ² ¹北茨城市立総合病院脳神経外科 ²筑波大学臨床医学系脳神経外科 ¹Department of Neurosurgery, Kitaibaraki Municipal General Hospital ²Department of Neurological Surgery, Institute of Clinical Medicine, University of Tsukuba キーワード： glioma capillary , ultrastructure , pinocytic vesicle , tight junction , fenestration Keyword: glioma capillary , ultrastructure , pinocytic vesicle , tight junction , fenestration pp.49-56

発行日 1991年1月1日 Published Date 1991/1/1

DOI https://doi.org/10.11477/mf.1406900149

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Abstract 文献概要
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　low grade astrocytomaおよび悪性神経膠腫の腫瘍辺縁部，内腔径10μm以下の血管の微細構造の比較から，神経膠腫血管の透過性に関する形態的特徴を，定量的に明らかにすることを目的とした。＜対象＞low grade astrocytoma 11例，28血管，悪性神経膠腫13例，30血管である。＜方法＞各血管につき，1）内皮細胞の細胞質1μm²あたりのpinocytic vesicle（PV）の数，2）junctionの異常（tight junctionの長さが0.25μm以下のjunction）の割合，3）fenestrationの有無を測定し，low grade astrocytoma，悪性神経膠腫の腫瘍血管の間で比較検討した。＜結果＞1）細胞質1μm²あたりのPVの数は，low grade astrocytomaで平均4.0±2.7個，悪性神経膠腫で平均8.1±5.0個で，悪性神経膠腫で有意に（p＜0.01）多かった。2）junctionの異常の割合は，low grade astrodytomaで10.3％，悪性神経膠腫で8.9％であり，違いは見られなかった。3）fenestrationを有する血管は見られなかった。＜結論＞神経膠腫の血管透過性の亢進に関する微細構造として，PVの増加が重要であり，junctionの異常，fenestrationの関与は少ない。PVの数はlow gradeastrocytomaでは正常と変わりなかったが，悪性神経膠腫では有意に増加していた。

In this study we investigated the ultrastructure of human glioma capillaries in operated sample of low grade astrocytomas and malignant gliomas. Electronmicrographs of a total of 58 vessels were analyzed with computer assisted morphometry for ultrastructural evidence of permeability routes. All of these vessels were present in the marginal area of tumors devoid of nocrosis, less than 10 micron in diameter and containing one nucleus at least on axial section. We found that : ( 1 ) The number of pinocytic vesicles was sinsificantly higher in capil-laries from malignant glioma (an average of 8.1 per 1 μm² cytoplasm) than those from low grade astrocytoma (an average of 4.0 per 1μm² cyto-plasm). In capillaries from malignant glioma, most of the pinocytic vesicles were arranged in the ab-luminal side of endothelium and some of them were fused each other. (2) Abnormal endothelial intercel-lular junctions which were defined as short tight junctions (≦0.25μm) were equally but infrequently seen in low grade astrocytomas and malignant gliomas. (3) Fenestrations in the endothelium were not seen. Therefore we suggest that the high vascu-lar permeability and resultant brain edema in malig-nant gliomas is likely to increased pinocytic vesicles and rare but abnormal inter endothelial junctions.