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Brain and Nerve No to Shinkei Volume 43, Issue 2 （February 1991）

Brain and Nerve 脳と神経 43巻2号（1991年2月発行）

Japanese English

原著

神経膠腫の腫瘍血管の微細構造—内皮細胞の増殖能に関する形態計測的検討 Ultrastructure of Glioma Vessel : Morphometric Study for Proliferative Potential of Endothelial Cell 高野晋吾 ¹ , 吉井与志彦 ¹ , 能勢忠男 ² Shingo Takano ¹ , Yoshihiko Yoshii ¹ , Tadao Nose ² ¹北茨城市立総合病院脳神経外科 ²筑波大学臨床医学系脳神経外科 ¹Department of Neurosurgery, Kitaibaraki Municipal General Hospital ²Department of Neurological Surgery, Institute of Clinical Medicine, University of Tsukuba キーワード： glioma capillary , ultrastructure , Weibel-Palade body Keyword: glioma capillary , ultrastructure , Weibel-Palade body pp.145-152

発行日 1991年2月1日 Published Date 1991/2/1

DOI https://doi.org/10.11477/mf.1406900159

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Abstract 文献概要
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　前回（脳神経43：49-56,1991）と同じ対象を用いて，今回は神経膠腫血管の内皮細胞の増殖能に関する形態的特徴を，明らかにすることを目的とした。

　〈方法〉各血管につき，1）血管内腔狭小度（LN：内腔面積に対する外周面積の比），2）基底膜の厚さ（BM），3）Weibel-Palade body（WPB）の出現頻度（WPB−1：全内皮細胞に対するWPBを含む内皮細胞の割合，WPB−2：一個の内皮細胞に含まれるWPBの数），4）核の形態的異常（NA：4段階にgrading），5）Mito—chondrial density（MIT）を測定し，low grade astrocytoma，悪性神経膠腫の腫瘍血管の間で比較検討した。〈結果および結論〉LNは悪性神経膠腫で28％，low grade astrocytomaで39％，以下同様にBMは2.9μm，1.2μm,WPB−1は34％，14％，WPB−2は0.92個，0.39個，NAはgrade 2.0, 1.3, MITは5.6％，4.2％であり両者の間で有意な違いが見られた。すなわち，悪性神経膠腫ではlow grade astrocytomaに比し，LNが強く，BMが厚く，WPBが高く，NAが強く，MITが多かった。以上より，LN, BM, WPB−1, WPB−2，NA, MITは内皮細胞の増殖能を表すパラメーターと考えられた。さらに，WPB−1は，悪性神経膠腫の早期再発例で44.3％と特に高く，内皮細胞の増殖能を表す特に重要なパラメーターと考えられた。

In this study we quantified the morphological abnor-malities of human glioma vasculature in operated sam-ple of low grade astrocytomas and malignant gliomas. Only those vessels with a diameter of less than 10 micron and containing one nucleus at least on axial section present in the marginal area of the tumors devoid of necrosis were subjected to the present study. A total of 58 vessels were analyzed with computer assisted morphometry for ultrastructural evidence of prolifer-ative potentials of endothelial cells.

5 specific features of the endothelial cells and/or the capilaries which were not related to the vascular perme-ability were assessed : (1) Degree of vascular luminal narrowing (LN : Ratio of luminal area to abluminal area), (2) Thickness of basemet membrane (BM), (3) Mean % ratio of the endothelial cells including Weibel-Palade bodies to whole endothelial cells (WPB-1) and the number of Weibel-Palade bodies in an endothelial cell section (WPB-2), (4) Irregularity of nuclear shape (NA : semiquantified grade 0 to 3), (5) Mitochondrial density (MIT).

We found that : (1) LN was significantly stronger in malignant glioma capillaries (MGC, 28%) than low grade astrocytoma capillaries (LAC, 39%), (2) BM was significantly thicker in MGC (2.9μm) than in LAC (1. 2μm), (3) WPB-1 and WPB-2 were significantly higher in MGC (34% and 0.92) than LAC (14% and 0.39), (4) NA was significantly higher in MGC (grade 2.1) than LAC (grade 1.3), (5) MIT was significantly higher in MGC (5.6%) than LAC (4.2%).

Therefore we suggest that LN, BM, WPB-1, WPB-2, NA and MIT are the parameters which represent the proliferative potential of endothelial cells in human gliomas. Especially, WPB-1 which was particularly high in malignant gliomas caused early recurrence not only gives information about the proliferative potentials of endothelial cells but also often a reliable index of malignancy.