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Japanese

THE INHIBITORY EFFECT OF ELASTASE ON CALCIUM INCREASE IN BRAIN AND SPINAL CORD OF RABBITS WITH ATHEROSCLEROSIS INDUCED BY CHOLESTEROL-RICH DIET Masayuki Yasui 1 , Ichiro Yano 1 , Hironobu Yoshida 2 , Fumio Yoshimasu 2 , Kiichiro Ota 3 , Akira Oshima 4 1Division of Neurological Diseases, Wakayama Medical College 2Department of Neuropsychiatry, Wakayama Medical College 3Laboratory Medicine, Wakayama Medical College 4The first Pathology, Wakayama Medical College pp.781-787
Published Date 1989/8/1
DOI https://doi.org/10.11477/mf.1406206365
  • Abstract
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The aim of present experiment was to investi-gate the decalcified effects of exogenous elastase in liver, kidney and central nervous system (CNS) of rabbits with atherosclerosis experimentally in-duced by the modified procedure of Kritchevsky et al. Twenty five male rabbits, weighing appro-ximately 2 kg, were divided into 6 groups. Animals were fed for 3 months with standard diet (group A), standard diet containing 1.5% cholesterol (group B) and 1. 5% cholesterol-rich diet plus intraperitoneal (ip) daily administration of elastase 450 EL. U/kg (group C). Another groups were kept for 6 months with standard diet (group D), standard diet containing O. 67% cholesterol (group E) and O. 67% cholesterol-rich diet plus same dose of elastase (group F). The rabbits treated with cholesterol-rich diet were confirmed to be induced atherosclerosis biochemically as well as histologi-cally. All groups were maintained under these conditions for experimental periods and allowed tap water. After 3 and 6 months, blood collected by cardiocentesis using ether anesthesia and then sacrificed to remove CNS and internal organs. Blood had stood for 1 hour at room temperature. Serum was separated by centrifugation at 3, 000 rpm for 10 min to determine total cholesterol, triglyceride, phospholipids, HDL-cholesterol, andso on. Calcium contents in the cerebral frontal lobe, cerebellum, pons, spinal cord, liver and kidney were measured by neutron activation analysis method. In this experiment the ameliora-tion of atherosclerosis by ip administration of elastase was ascertained. In rabbits given chole-sterol-rich diet, calcium content in CNS tissues was higher than that of another tissues andparalleled to a rise of serum cholesterol level. Calcium contents in cerebellum and spinal cord in group F significantly decreased as compared with those of group E. These results indicate that exogenous elastase has decalcified effects on the calcium deposition in CNS tissues of rabbits with experimental atherosclerosis.


Copyright © 1989, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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