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MORPHOLOGICAL STUDIES ON ESTROGEN-INDUCED PRL CELL HYPERPLASIA IN RAT PITUITARY GLAND: IMMUNOCYTOCHEMICAL AND SCANNING ELECTRON MICROSCOPIC STUDIES Hirotaka Inagaki 1 , Tomokatsu Hori 1 1Department of Neurosurgery, Tottori University School of Medicine pp.593-601
Published Date 1989/6/1
DOI https://doi.org/10.11477/mf.1406206335
  • Abstract
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It is well known that the tumorous lesion in rat anterior pituitary gland occurres after continuous administration of estrogen, which is mainly com-posed of PRL secreting cells. In this study the changes in rat PRL cells by estrogen treatment were studied histologically and immunocytoche-mically. Furthermore, intracellular structures inPRL cells were observed by high resolution scan-ning electron microscopy using the aldehyde prefix osmium-DMSO-osmium method. These rat pitui-tary tumors were established in Fischer-344 female rats by weekly injections of 2. 5 mg estradiol valerate. The estrogen-induced, autonomous and transplantable rat pituitary tumors (MtT/F 84) were also studied in the same manner and the findings were compared with estrogen-induced tumors. The increase of weight of pituitary gland and of serum PRL level correlated significantly with the number of estrogen administration.

On the basis of histological findings, estrogen-induced pituitary tumors can be classified into two different stages, that is, hypertrophic hyperplasia and adenomatous hyperplasia. The former corre-sponds to the period unitil 6 weeks after the be-ginning of estrogen treatment and the latter to the period thereafter. In the former, PRL cells were significantly increased in number and volume, and were diffusely immunoreactive for PRL in the cytoplasm. Cell atypism was rarely found. Theextent of acidphil staining was well preserved in hematoxilin-eosing staining. Ultrastructurally, organelles were well developed, especially rough endoplasmic reticulum showed whorl formation. Immature, irregularly shaped secretory granules were frequently observed within the Golgi area. Mature granules were richly stored at the peri-phery of the cell. The stage of adenomatous hyperplasia, on the other hand, was characterized by the obscurity of acinar pattern, the prominent cell atypism and mitosis, and the increased numbers of chromophobic cells. Electron microscopically, secretory granules were sparsely distributed in con-trasting to organelles which were more strongly developed. These findings in adenomatous hyper-plasia resembled to those of the MtT/F 84 tumor cell to some extent.

The implication of the study of estrogen-induced rat pituitary tumor as an experimental model for human PRLnoma was discussed.


Copyright © 1989, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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