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Brain and Nerve No to Shinkei Volume 39, Issue 12 （December 1987）

Brain and Nerve 脳と神経 39巻12号（1987年12月発行）

Japanese English

原著

In vivoボルタメトリー法による脳内モノアミン代謝の測定—基礎実験と病態モデルへの応用 MEASUREMENTS OF MONOAMINE NEUROTRANSMITTER METABOLISM BY IN VIVO VOLTAMMETRY 小倉浩一郎 ^1,2 , 渋谷正人 ¹ , 金森雅彦 ¹ , 鈴木善男 ¹ , 原田努 ¹ , 岡田知久 ¹ , 田ノ井千春 ¹ , 景山直樹 ¹ Koichiro Ogura ^1,2 , Masato Shibuya ¹ , Masahiko Kanamori ¹ , Yoshio Suzuki ¹ , Tsutomu Harada ¹ , Tomohisa Okada ¹ , Chiharu Tanoi ¹ , Naoki Kageyama ¹ ¹名古屋大学医学部脳神経外科 ²現籍：市立四日市病院脳神経外科 ¹Department of Neurosurgery, Nagoya University School of Medicine pp.1181-1187

発行日 1987年12月1日 Published Date 1987/12/1

DOI https://doi.org/10.11477/mf.1406206030

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Abstract 文献概要
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　抄録　In vivoボルタメトリー（IVV）法による基礎実験を行なうとともに，心停止時の脳内モノアミン代謝を測定した。in vitroにおける3,4—dihydroxyphenylacetic acid （DOPAC）および5-hydroxyindole-acetic acid　（5-HIAA）のリン酸緩衝溶液（PBS）を用いた基礎実験の後，in vivoではラット線条体における各種薬物投与のDOPAC, 5-HIAAに及ぼす影響を，更にKClの静脈内投与による心停止時のDOPAC, 5-HIAAの変動を経時的に測定した。in vitroでは，DOPACは5×10⁻⁶〜10⁻⁴　M, 5-HIAAは10⁻⁶〜10⁻⁴Mの範囲において直線状の比例関係をもちそれぞれのピークが測定された。またPBS溶液のpHが1低下すると，それぞれのピークの出現が約30mV遅れることも分かった。in vivoにおいては，pargyline, haloperidol, probenecid等のモノアミン代謝に影響する薬物の投与によりそれぞれに相応するDOPAC, 5-HIAAの変化を経時的に測定することが出来た。心停止後にはDOPAC, 5-HIAAともにその早期に急激な増加を示し，脳虚血急性期におけるドーパミン，セロトニンの高度な放出亢進が示唆された。

In vivo voltammetry is an electrical detection method by which dopamine and serotonine meta-bolism can be estimated in the brain of animals. In the present report preliminary and basic ex-periment were performed before its apllication to ischemic brain.

Firstly, using voltammetry DPV-5 system in vitro, concentration/amplitude curve was obtained with various concentration of 3,4-dihydroxyphenyl-acetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5-HIAA) diluted in phosphate buffer (pH 7.4) . DOPAC appeared at + 150 mV (peak 2)while 5-HIAA appeared at +300 mV (peak 3). A linear relation was obtained in the concentra-tions ranging 5×10^-6~10^-4 M for DOPAC and 10-6~10-4 M for 5-HIAA. While a delay in oxida-tion potential was noticed for both peaks 2 and 3 by about 30 mV when the pH of solution was decreased by 1.

Secondly the DPV-5 system was applied in vivo to the rat striatum. Peak 2 was observed at +150 mV which was increased by intraperitoneal injec-tion of haloperidol, a dopamine receptor blocker and decreased by pargyline, a monoamine oxidase inhibitor. While peak 3 was observed at +300 mV and increased after probenecid and decreasedby pargyline, which suggest that peak 2 and 3 correspond to DOPAC and 5-HIAA respectively.

Thirdly DPV-5 system was applied to the rat striatum and rat's heart was arrested by KC1 in-jection as a model of extreme brain ischemia. Shortly after cardiac arrest the heights of peak 2 was significantly increased to 600~900% while the increase of peak 3 was not significant, both of which subsided gradually toward 30 minutes after arrest. These findings suggest that DPV-5 system can be used for ischemic brain in order to measure changes of dopamine and serotonine metabolism.