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Brain and Nerve No to Shinkei Volume 44, Issue 4 （April 1992）

Brain and Nerve 脳と神経 44巻4号（1992年4月発行）

Japanese English

原著

実験的ラットくも膜下出血モデルにおける脳幹部モノアミン神経機能の変動—in vivo microdialysis法による検討 Changes of Monoamine Metabolites Measured by In Vivo Microdialysis in the Brainstem Following Experimental Subarachnoid Hemorrhage in Rats 平田勝俊 ¹ Katsutoshi Hirata ¹ ¹長崎大学医学部脳神経外科 ¹Department of Neurosurgery, Nagasaki University, School of Medicine キーワード： subarachnoid hemorrhage , catecholaminergic neuron , nucleus tractus solitarius（NTS） , liquid chromatography , in vivo microdialysis Keyword: subarachnoid hemorrhage , catecholaminergic neuron , nucleus tractus solitarius（NTS） , liquid chromatography , in vivo microdialysis pp.331-336

発行日 1992年4月1日 Published Date 1992/4/1

DOI https://doi.org/10.11477/mf.1406900320

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Abstract 文献概要
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　ラットくも膜下出血モデルを用いて，くも膜下出血後に起こる脳幹部モノアミンニューロンの機能をin vivo microdialysis法によりその代謝産物を測定することで検討した。プローブを挿入した延髄孤束核（NTS）から検出できるモノアミン代謝産物は，5-hydroxyindoleacetic acid（5-HIAA），homovanillic acid（HVA），3,4-dihydroxyphenylacetic acid（DOPAC）が主体であった。くも膜下出血後0〜120分の急性期の変動では，一過性に代謝産物が上昇し，脳幹部圧迫に伴う虚血性変化を反映するものと思われた。一方，経日的変化をmonoamine oxidase阻害剤投与後の代謝産物の消失速度でみると，くも膜下出血後2日目が最も速く，その後回復する傾向にあった。特に，DOPACのearly compartmentで有意に消失速度が速くなることより，norepinephrine系では細胞体に比べ軸索から神経終末にかけての障害が強いことが示唆された。

We investigated the function of monoaminergic neuron in the brainstem by measuring its metabolites using in vivo microdialysis following experimental subarachnoid hemorrhage in rats. Dialysis probe was implanted into the nucleus tractus solitarius (NTS) and continuous perfusion was then started. The perfusates were collected every 10-20 minutes and assayed by high-perfor-mance liquid chromatography (HPLC) with electro-chemical detection (ECD).

The main monoamine metabolites in extracel-lular space measured in NTS were 3 ,4-dihydroxy-phenylacetic acid (DOPAC), homovanillic acid (HVA), and 5-hydroxyindoleacetic acid (5-HIAA). The extracellular content of DOPAC was abruptry increased after cisternal autologous blood (0.3m1) injection, reached a peak at 20-40 minutes, and then decreased over 120 minutes. The content of HVA and 5-HIAA changed as well as DOPAC. These results showed non-specific response for is-chemia of the brainstem, because the similar changes were seen after cisternal saline injection.

The disapperance rate of monoamine metabolites after pargyline administration (75 mg/kg, i. p.) at various time periods after cisternal blood injection was rapidest at 2 days after SAH and recovered gradually. In particular the decline curve of DOPAC consisted of two compartments and early compartment was disturved more severely than late compartment. These results indicate that the func-tional disturbance of nerve terminals is more severe than nerve cell body in adrenergic neurons.