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BIOCHEMICAL STUDIES OF THE CEREBRAL ISCHEMIA IN THE RAT: CHANGES IN CEREBRAL FREE Amino ACIDS, CATECHOLAMINES AND URIC ACID Daisei Kaneko 1 1Department of Neurosurgery, Jikei University School of Medicine pp.253-258
Published Date 1986/3/1
DOI https://doi.org/10.11477/mf.1406205675
  • Abstract
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Changes in cerebral free amino acids, cate-cholamines and uric acid levels were explored for up to 7 days after cerebral ischemia in the rat. Fifty male Sprague-Dawley rats were subjected to occlusion of the middle cerebral artery on the olfactory tract, under halothane anesthesia.

The animals were decapitated at 2, 4, 6, 12, 24 hours and 2, 3, 5, 7 days after the surgery, respec-tively. The brains were rapidly removed. The cerebral hemispheres were divided into right and left halves, and homogenized in sulfosalicylic acid solution. Free amino acids were analyzed by color-metric method. Cathecholamines and uric acid were analyzed by high-performance liquid chro-matography. Each parameters were measured both on the ischemic and contralateral hemi-spheres. The time course of changes in each para-meters were observed by means of the ratio, which is the value of ischemic side divided by that of contralateral side.

1. Free amino acids a) Dicarboxylic group ; Decreases in glutamate and increases in glutamine suggest one aspect of detoxication of ammonia within the ischemia tissue. b) Monocarboxylic group ; GABA, glycine, alanine were increased in early ischemic state, and gradually lowered to the normal values. These suggest the impair-ment of tricarboxylic acid (TCA) cycle in the ischemic tissus, since these anino acids are closely related to TCA cycle. c ) Essential amino acids, except for tryptophan, were increased until the end of study. These increases suggest the utiliza-tion of essential amino acids for protein synthesis might be disturbed in the ischemic tissues. 2. Catecholamines and precursors ; Norepinephrine and dopamine were lowered gradually. On the other hand, phenylalanine and tyrosine were in-creased during ischemia. This result suggests the impaired biosynthesis of catecholamines followed by disutilization of phenylalanine and tyrosine in the ischemic tissue. 3. Uric acid increased gra-dually, reached to three-hold of control value at the end of study. It is known that free radicals are produced in the circuitry of uric acid syn-thesis. Then, this result suggests some degree of derangement in Blood-Brain-Barrier following the induction of free radical reaction in the infarcted lesion.


Copyright © 1986, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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