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LOCALIZATION OF SOMATOSTATIN IN HUMAN BRAIN TUMORS Hiroshi Hara 1 , Toshiaki Moriki 1 , Masahiro Miyao 1 , Machiko Hashimoto 1 , Toshiko Yamane 2 1Department of Pathology, Kochi Medical School 2Department of Pathology, Public Health Institute of Kochi Prefecture pp.553-558
Published Date 1983/6/1
DOI https://doi.org/10.11477/mf.1406205134
  • Abstract
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Although a number of reports demonstrated that somatostatin is distributed widely in the central nervous system, the presence and distribu-tion of somatostatin in the human brain tumors has not been well documented yet. In this report,the immunohistochemical localization of somato-statin in the human brain tumors was studied using the peroxidase-antiperoxidase (PAP) method and compared with the distribution of insulin, glucagon and neurophysin. The main purposes of this study were to analyze the distribution pattern of immunostaining in different types of glial neoplasms.

Fourty-five cases of human intracranial and spinal cord tumors (27 gliomas, 12 meningiomas and six other non-glial tumors) selected mostly from surgical materials were examined for the localization of these peptides. Dako PAP KIT (Dako Corporation) was used for immunostaining and applied to 3 /tm thick sections cut from formalin-fixed, paraffin-embedded tissue.

The results were as follows:

1) A positive immunostaining for somatostatin, insulin and glucagon was found in most of the tumor cells with abundant cytoplasm in astrocyt-omas, particularly in gemistocytic astrocytomas. The immunostaining was prominent in the perikarya and in the cell processes. The fibrillarybackground was also diffusely stained.

2) In glioblastoma multiforme the cytoplasm of gemistocytic cells, large bizarre cells, and fusiform cells exhibited immunoreactivities of somatostatin, insulin and glucagon. Immunostaining for these peptides was less prominent in glioblast-omas than in gemistocytic astrocytomas. Small cells with scanty cytoplasm showed no immuno-staining.

3) Positive reaction to neurophysin was observed only moderately in occasional tumor cells of astrocytomas and of glioblastomas.

4) Ependymoma, oligodendrogliomas and me-dulloblastomas were negative for all four of these peptides examined. However, reactive astrocytes found around tumors showed positive staining for somatostatin, insulin and glucagon. Reaction to neurophysin was negative.

5) Meningiomas of meningotheliomatous type showed positive staining for somatostatin, insulin and glucagon. Neurilemmomas were negative in immunostain for all these peptides.


Copyright © 1983, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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