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Japanese

IMMUNOHISTOLOGICAL DIAGNOSIS OF HUMAN AND EXPERIMENTAL BRAIN TUMORS USING ANTISERUM TO ASTROPROTEIN Toshiki Yoshimine 1 , Yukitaka Ushio 2 , Toru Hayakawa 1 , Kazuyoshi Morimoto 2 , Takesada Mori 3 , Heitaro Mogami 2 1Department of Neurosurgery, The Center for Adult Diseases 2Department of Neurosurgery, Osaka University Medical School 3Second Department of Surgery, Osaka University Medical School pp.85-93
Published Date 1979/1/1
DOI https://doi.org/10.11477/mf.1406204361
  • Abstract
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Human and ethylnitrosourea-induced rat brain tumors were studied by the indirect immuno-fluorescence technique using antiserum to astro-protein (an astrocyte-astrocytoma cell specific cerebroprotein) with a view to applying the method to the immunohistological diagnosis of the tumors.

Specific fluorescence seen in astrocytes and astro-sytoma cells was proved contributory to the diagnosis of human astrocytomas and glioblastomas. On glioblastoma preparations, sarcomatous regions were easily identified by the lack of fluorescing components.

Experimental brain tumors of animals are often difficult to be diagnosed by the conventional stainings. No positive stain was seen by Mallory's PTAH stain in all the types of the induced ratbrain tumors in the present series, whereas the immunofluorescence technique disclosed valuable differences between the tumors. The rationale of using antiserum to human astroprotein in rat specimens is explained by the immunological cross-reactivity of the antigens between human and other mammalian species. One type of the tumor showed overwhelming fluorescing cells and was diagnosed as astrocytoma. Another tumor was diagnosed as oligodendroglioma because of the negative results on the immunofluorescence study. HE stain of the third type of the experimental tumor showed a few, exceptionally large, bizarre cells intermingled with other predominating, smaller, uniform cells. On the immunofluorescence study, only the eccentric cells were fluoresced. These findings were interpreted as those of oligo-dendroglioma with some astrocytic components or of mixed glioma of oligodendroglioma and astro-ytoma.

One coronal section of the grossly normal brain of a rat in this series had a small, well circumscribed lesion of cellular proliferation. On the immuno-fluorescence study, the cytoplasm of most of the cells concerned was fluoresced and the shapes and arrangements of the cell bodies and processes were examined in detail. The nature of the lesion was considered as one of the pre- or early neoplastic changes induced by the agent.

Immunohistological examinations using antiserum to astroprotein were proved useful in the histo-pathological diagnosis of human as well as rat brain tumors. Further study with the method will be expected to contribute to the identification of tumors with diagnostic difficulties or whose origins are under debate.


Copyright © 1979, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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