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Brain and Nerve No to Shinkei Volume 39, Issue 6 （June 1987）

Brain and Nerve 脳と神経 39巻6号（1987年6月発行）

Japanese English

原著

ヒト脳腫瘍における中間径filamentの発現様式—Vimentinおよびastroprotein （GFAP）による免疫組織化学的研究 IMMUNOHISTOCHEMICAL STUDY OF HUMAN BRAIN TUMORS WITH VIMENTIN AND ASTROPROTEIN (GFAP) 丸野元彦 ¹ , 吉峰俊樹 ² , 生塩之敬 ² , 早川徹 ² , 有田憲生 ² , 尾藤昭二 ¹ , 最上平太郎 ² Motohiko Maruno ¹ , Toshiki Yoshimine ² , Yukitaka Ushio ² , Toru Hayakawa ² , Jamshid Jamshidi ² , Norio Arita ² , Shoji Bitoh ¹ , Heitaro Mogami ² ¹大阪厚生年金病院脳神経外科 ²大阪大学医学部脳神経外科 ¹Department of Neurosurgery, Osaka Kouseinenkin Hospital ²Department of Neurosurgery, Osaka University Medical School pp.579-585

発行日 1987年6月1日 Published Date 1987/6/1

DOI https://doi.org/10.11477/mf.1406205926

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Abstract 文献概要
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　抄録　ヒト脳腫蕩組織におけるastroprotein （GFAP）およびvimentinの発現様式を検討するため，それぞれに対する特異抗体を用いた免疫組織化学的検索を行った。間葉系腫瘍であるmenigiomaは常にastroprotein （GFAP）陰性，vimentin陽性を示した。良性のastrocytoma cellはastroprotein （GFAP）陽性，vimentin軽度陽性を示したが，anaplastic astrocytomaではastroprotein （GFAP）陽性細胞は減少し，逆にvimentin陽性細胞が増加する傾向が認められた。Glioblastomaではastroprotefn （GFAP）陽性細胞がさらに減少したが，vimentin陽性細胞が優勢の場合，あるいは逆にvimentin陰性細胞が優勢の場合とさまざまであった。すなわち，astrocytoma-glioblastoma系腫瘍では組織学的悪性度の増加に伴いglial filamentにかわりvimentin filamentが主体を占めるようになるが，さらに脱分化が著しくなると中間径filamentの発現そのものが抑制される可能性が示唆された。

Distributions of two different subclasses of in-termediate filaments, vimentin and glial filaments, were studied immunohistochemically in human brain tumors using specific antiserum to each pro-tein subunit, vimentin and astroprotein (GFAP).Surgical specimens (5 meningiomas, 4 ependymo-mas, 5 benign astrocytomas, 5 anaplastic astrocy-tomas and 7 glioblastomas) were fixed in 95% ethanol or ethanol-acidic acid (95 : 5) and embed-ded in paraffin. Avidin biotin peroxidase-complex (ABC) method (Vectastain®) was carried out on 6 micron-thick paraffin sections. All meningioma cells were negative for astroprotein (GFAP) and positive for vimentin. Ependymoma cells showed various patterns of immunoreaction for astropro-tein (GFAP) but were invariably positive for vimentin. In benign astrocytomas, many cells (or cell body and processes) were positive for astro-protein (GFAP). Immunoreaction for vimentin was, however, less frequent and intense. In anaplastic astrocytomas, population of astroprotein (GFAP)-positive cells decreased and vimentin-positive cells increased. Astroprotein (GFAP)-positive cells were further decreased in glioblastomas and the popula-tion of vimentin-positive cells varied among tis-sues. The present study suggests that the anapla-stic change of astrocytoma cells were associated with decreased expression of glial filaments and increased expression of vimentin filaments. It was also suggests that the expression of both interme-diated filaments may be suppressed in highly-malignant glial tumor cells.