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Japanese

THE EFFECT OF FREEZING AND FORMALIN PRESERVATION ON LIPID, FATTY ACID COMPOSITION AND GANGLIOSIDE PATTERN IN RAT BRAINS Hideyuki Uchimura 1 , Masashi Saito 1 , Akira Mukai 1 , Hidebumi Hazama 2 1Hizen National Mental Hospital 2Department of Neuropsychiatry, Faculty of Medicine, Kyushu University pp.83-89
Published Date 1972/1/1
DOI https://doi.org/10.11477/mf.1406203048
  • Abstract
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Lipid, fatty acid composition and ganglioside pat-tern in adult rat brains were investigated afterpreservation in following conditions : 1) kept atroom temperature (26℃) for 24 hr., 2) Stored in10% formalin solution for 3, 6 and 12 months, 3)frozen at-20℃ for 3, 6 and 12 months. Thinlayer chromatography and gas-liquid chromato-graphy were used for analysis.

The results were compared with those of freshadult rat brains as follows :

1) Post mordem changes :

Slight increase of cholesterol, and decrease ofsulfatide and phosphatidyl serine were observed.However, cerebroside sphingomyelin, phosphatidylethanolamine and phosphatidyl choline and gan-glioside pattern remained unchanged.

Regarding fatty acid composition of each lipid,decrease of long chain fatty acids in cerebrosideand slight changes of fatty acid composition insulfatide and phosphatidyl serine were found.

2) Effect of formalin preservation :

In the formalin preserved brain for 3 months,phosphatidyl ethanolamine disappeared and sulfatideslightly decreased. Moreover, in the preservedbrain for one year, phosphatidyl serine and phos-phatidyl choline slightly decreased. While choles-terol, cerebroside and sphingomyelin remained un-changed.

Moreover, changes of fatty acid composition inall lipids except phosphatidyl choline were observed.Particulary, long chain fatty acids in cerebrosidemarkedly decreased.

On the other hand, marked changes of ganglio-side pattern were found. G 1, a trisialoganglioside,disappeared in the formalin preserved brain for 3months, and G 1, G 2 and G 3 disappeared and onlyG 4, a monosialoganglioside, was found in the pre-served brain for one year.

3) Effect of freezing :

Lipid, fatty acid composition and gangliosidepattern in frozen brain for one year remained un-changed. However, decrease of long chain fattyacids in cerebroside and changes of fatty acidcomposition in sulfatide were observed.

From these results, it can be concluded as fol-lows : Formalin preserved brain is not suitable forchemical analysis of lipid, fatty acid compositionand ganglioside pattern. On the other hand, frozenbrain can be available enough even if after frozenfor one year.


Copyright © 1972, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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