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Stomach and Intestine(Tokyo) Volume 30, Issue 2 （February 1995）

胃と腸 30巻2号（1995年2月発行）

Japanese English

今月の主題表面型大腸癌の発育と経過

主題研究

大腸表面型腫瘍におけるDCC遺伝子コドン201の多様性に関する検討 DCC Gene Codon 201^Arg/Gly Polymorphism in Flat Type Colorectal Tumors 延沢利江子 ^1,2 , 青山伸郎 ¹ , 本迫洋一郎 ¹ , 春日雅人 ¹ , 長廻紘 ³ , 藤盛孝博 ² , 前田盛 ² Rieko Nobusawa ^1,2 , Nobuo Aoyama ¹ , Kou Nagasako ³ , Takahiro Fujimori ² , Sakan Maeda ² ¹神戸大学医学部第2内科 ²神戸大学医学部第2病理 ³東京女子医科大学消化器病センター内科 ¹The Second Department of Internal Medicine, Kobe University of Medicine ²The Second Department of Pathology, Kobe University School of Medicine ³Institute of Gastroenterology, Tokyo Women's Medical College キーワード：大腸表面型腫瘍 , DCC遺伝子 , 癌遺伝子 , 癌抑制遺伝子 Keyword: 大腸表面型腫瘍 , DCC遺伝子 , 癌遺伝子 , 癌抑制遺伝子 pp.207-212

発行日 1995年2月25日 Published Date 1995/2/25

DOI https://doi.org/10.11477/mf.1403105317

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Abstract 文献概要
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要旨　DCC遺伝子コドン201（Arg/Gly）の多様性を表面型腫瘍37例を対象とし，隆起型腫瘍81例，進行癌54例と比較した．腫瘍高が正常粘膜以下のものを表面型とした．末梢血・正常粘膜に201^Glyフェノタイプを認めたものは，表面型ではlow grade dysplasia（low）67％，high grade dysplasia（high）64％，sm carcinoma64％と異型度にかかわらず高率であった．隆起型ではlow20％，high48％，sm carcinoma50％，進行癌では54％，対照は17％であった．末梢血・正常粘膜に201^Arg/Glyヘテロ接合体を有する例で，腫瘍部に片対立遺伝子の欠失を認めた6例のうち5例は201^Argが欠失し，201^Glyが残存していた．以上から，DCC遺伝子コドン201^Glyフェノタイプは表面型腫瘍にも密接に関与していることが示唆された．

　Codon 201^Arg/Gly polymorphism in the DCC (deleted in colorectal carcinoma) gene was analyzed in 37 flat type colorectal tumors and compared with that of 81 polypoid type tumors and 54 advanced carcinomas. The flat type was defined microscopically as one whose height was less than that of the surrounding normal mucosa, and others were included in the polypoid type. The phenotypic frequencies of the codon 201^Gly in flat type tumors were 67% (6/9), 64% (9/14), 64% (9/14) in low grade dysplasia (low), high grade dysplasia (high), carcinoma invading the submucosa (sm carcinoma), respectively. There were high frequencies of the codon 201^Gly phenotype in flat type tumors as well as in polypoid type high 48% (16/33), sm carcinoma 50% (14/28) and advanced carcinoma 54% (29/54), but flat type tumors showed significantly higher frequencies of codon 201^Gly phenotype than polypoid type low 20% (4/20) and controls (who had neither colorectal tumor nor a family history of colon cancer) 17% (5/30) . The codon 201 phenotype in the colorectal tumor tissues did not differ from that in the surrounding normal mucosa or in peripheral blood leukocytes, except that loss of heterozeigosity (LOH) occurred in the six cases with carcinoma. Five of them with LOH showed the codon 201^Gly allele deletion and one patient showed the codon 201^Gly allele deletion. These results suggest that the codon 201^Gly phenotype in the DCC gene is not only a useful genetic marker for malignant colorectal tumors but that it is closely related to flat type colorectal tumors.