マウス脈絡叢上皮細胞のグリコゲン（II）—両側上頸神経節除去，両側内側前脳束破壊および両側副腎除去による影響

GLYCOGEN IN EPITHELIAL CELLS OF THE CHOROID PLEXUS OF THE MOUSE (II). : EFFECTS OF SUPERIOR CERVICAL GANGLIONECTOMY, LESION OF THE MEDIAL FOREBRAIN BUNDLE AND ADRENALECTOMY

I．はじめに

　成熟した哺乳動物の脈絡叢上皮細胞には，一般にグリコゲンはみとめられない（Schaltenbrand, 1955^1）；Kappers, 1958^2）；　TennysonとPappas, 1961^3）；　NetzkyとShuangshoti, 1970^4））。このことはマウスにおいても例外でなく，生後約2週以後脈絡叢上皮細胞は組織化学的・電子顕微鏡的にみとめうるグリコゲンを欠く（鈴木1972^5））。しかしながら，さきに報告したように，成熟マウスにレセルピンを投与すると，その脈絡叢上皮細胞にはきわめて多量のグリコゲンが発現する（SuzukiとIto，1974^6）；鈴木，1974^7））。このようなレセルピンによるグリコゲンの発現は予めモノアミン酸化酵素抑制剤を投与すると起こらない。このことから，私どもは脈絡叢上皮細胞におけるグリコゲンの発現がモノアミン量の変化に関与することを推測した。本研究では，その関与するモノアミンの由来を明らかにするために，上頸神経節除去，内側前脳束破壊および副腎除去の影響を検索した。

Effects of superior cervical ganglionectomy, lesion of the medial forebrain bundle and adrenalectomy on glycogen content in the choroidal epithelial cells of adult mice were studied histochemically and electron-microscopically.

Following bilateral superior cervical ganglio-nectomy, small amounts of glycogen granules often appeared in the choroidal epithelial cells of the lateral and fourth ventricles at 3 hours after sur-gery. Thereafter glycogen gradually increased in amount until its content attained maximum at 6-9 hours. However it began to decrease at 12 hours and had disappeared at 24 hours and later. In the choroidal epithelium of the third ventricle, only smaller amounts of glycogen appeared later and disappeared earlier than in the lateral and fourth ventricles. In mice ganglionectomized, reserpine could induce accumulation of large amounts of gly-cogen in the choroidal epithelial cells.

Following bilateral lesion of the medial forebrain bundle, small amounts of glycogen were observed in the choroidal epithelium of the lateral ventricle at 3 hours and in that of the third ventricle at 6 hours. Glycogen gradually increased in amount and reached maximum at 12 hours. It still remained only in small amounts at 24 hours, but disappeared at 48 hours. In the choroidal epithelium of the fourth ventricle, on the other hand, no glycogen appeared throughout the entire course examined.

Following bilateral adrenalectomy, no glycogen accumulation was detected in the choroidal epithelial cells thus far examined. In adrenalectomized mice, however, administration of reserpine induced a strik-ing increase in glycogen in the epithelium of each choroid plexus.

Neither unilateral sympathetic ganglionectomy, nor unilateral lesion of the forebrain bundle, nor partial lesion of the frontal lobe of the brain and nor sham operation caused glycogen appearance in the choroid epithelial cells.