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Measurement of PCNA Labeling Index in Astrocytic Tumors Takaaki BEPPU 1 , Hiroshi ARAI 1 , Haruyuki KANAYA 2 , Kousuke SASAKI 1 1Department of Pathology, Iwate Medical University 2Department of Neurosurgery, Iwate Medical University Keyword: Astrocytic tumors , PCNA , Immunohistochemistry pp.1255-1259
Published Date 1992/12/10
DOI https://doi.org/10.11477/mf.1436900568
  • Abstract
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PCNA (proliferating cell nuclear antigen) is said to be present specifically in the nucleus of proliferating cells. The PCNA labeling index (PCNA LI) of astrocytic tumors was measured and compared with histological types or prognosis. The specimens from 44 patients were fixed in a 10% formalin solution, and embedded in paraf-fin. The 3 pm-sections were stained immunohistochemi-cally with anti-PCNA monoclonal antibody (PCIO, Novocastra) using an ABC method. The percentage of PCNA-positive-cells was determined by counting 2000 cells, and identified as PCNA LI.

All of the PCNA-positive-cells showed diffuse nucleo-plasmic staining. The averages of PCNA LIs in each pathological type we Although differences in averages of PCNA LIs among pilocytic, gemistocytic, fibrillary astrocytoma were not significant, there was a significant difference between anaplastic astrocytoma and glioblastoma. The relationship between PCNA LIs and the prognoses for 43 patients was studied. Forty-three patients were classi-fied into 3 groups (over 22%, 7 to less than 22%, and less than 7%) according to PCNA LIs. The survival data in the 3 groups were analyzed, and differed significantly in the survival rates. Furthermore, twenty-three patients of anaplastic astrocytoma and glioblastoma were classified into two groups (over 22% and less than 22%). Likewise, the two groups differed significantly.

In summary, pathological type and prognosis were closely related to PCNA LI in astrocytic tumors. There-fore, we thought measurement of PCNA LI would make it more possible to analyze clinically the proliferating activity of astrocytic tumors, and to care for patients more effectively.re calculated and evaluated statisti-cally.


Copyright © 1992, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1251 印刷版ISSN 0301-2603 医学書院

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