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Analysis of proliferating cell nuclear antigen (PCNA) expression in brain tumors Yasumasa HONJHO 1 , Takahiro TUCHIDA 2 , Takashi FUJIWARA 1 , Seigo NAGAO 1 1Department of Neurological Surgery, Kagawa Medical School 2Department of Neurological Surgery, Kansai Medical School Keyword: PCNA , immunoblotting , immunohistochemistry , brain tumor pp.143-148
Published Date 1996/2/10
DOI https://doi.org/10.11477/mf.1436901158
  • Abstract
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The expression of PCNA in brain tumor cells was measured in vitro and in situ by using an electrophore-tical immunoblotting method and an immunohistoche-mical staining method. In synchronized C6 rat glioma cells, PCNA were almost parallel to the S phase, although the small amounts of PCNA were expressed in G1 and G2 M phases. In astrocytic tumors from op-erative tissues, immunohistochemical PCNA positive rate increased significantly with increasing tumor grade. PCNA positive rate of recurrent meningiomas was also significantly higher than that in nonrecurrent meningiomas. These findings suggest that the PCNA is associated with the cell cycle, especially the S phase and immunohistochemical staining of PCNA is useful to evaluate the proliferating activity of a brain tumor. Immunoblotting method would also be helpful for the exact analysis of proliferating activity in brain tumor cells.


Copyright © 1996, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1251 印刷版ISSN 0301-2603 医学書院

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