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Rabies Synpossium on Fluorescent Antibody Technic and Viral Encephalornyelitiden Sugito Ohtani 1 1Depart. of Internal Medicine, Institute for Infectious Diseases, Univ. of Tokyo pp.5-12
Published Date 1965/3/25
DOI https://doi.org/10.11477/mf.1431904146
  • Abstract
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 The present attempts using the fluorescent antibody technique are focused to investigate thesimplified features of virus propagation in rabiesstreet virus encephalomyelitis and to clarifythe varieties of replication of rabies virus inthe various cells of suckling mice. The strainof street rabies virus originally isolated from aclog with natural infection in Tokyo area 1952were used. The rabies virus maintained theability of producing Negri bodies in the hippo-campus of young mice. Seven sucklings of 2or 3 days old of the DD mouse strain wereinoculated intramuscularly (M. Masseter dextra) with about 103 LD50 of the virus. Themice manifested typical signs of furious rabies8 days after inoculation and subsequentlygeneral paralysis for 2 Or 3 days before death. These animals were stunned and decapitatedat immediately frozen at -70℃ The gamma-globulin fractionated by means of the Kendall'method from the anti-rabies hyperimmunerabbit sera was conjugated with fluoresceinisothiocyanate by the method of Marshall at al. and store d in frozen. The conjugates wereabsorbed before use with acetone-dried powdered mixture of mouse brain and liver preparedaccording to the method of Coons et al. Freshfrozen sections of about 8 microns in thicknesswere prepared in a cryostat and fixed in acetoneat C for 1 minute. Each preparation was covered with the conjugate 1 : 20 in saline solutionfor 24 hours at 4℃. After washing in salinesolution, the preparations were mounted inElvanol and examined under the fluoresce ntmicroscope.

 The hosts in the rabies infection are salivarygland cell and nerve cell, in later cell the virusantigen is distributed in the process as wellthe cytoplasm. In the ganglia of both cranialand spinal nerve, brain stem and diencephalon, rabies virus antigen appears at an earlier stageof infection and incubation period of infection isshorter at the side of inoculation. Fine particlesof rabies virus antigen in acinar cells of the salivary gland firstly appears at the stage of 3 claysinfection, gradullya aggregate in the cellularperiphery of allsarrounding the auinal spaces.


Copyright © 1965, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 1882-1243 印刷版ISSN 0001-8724 医学書院

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