雑誌文献を検索します。書籍を検索する際には「書籍検索」を選択してください。

検索

書誌情報 詳細検索 by 医中誌

Japanese

Poliomyelitis: Symposium on Fluorescent Antibody Technic and Viral Encephalomyelitiden Nobuyuki Uchida 1 1Department of Pathology, National Institute of Health pp.13-22
Published Date 1965/3/25
DOI https://doi.org/10.11477/mf.1431904147
  • Abstract
  • Look Inside

 Fluorescent antibody technic (FAT) was used todemonstrate the viral antigen in the tissues of monkeys and mice infected with polio virus.

 Two ml of Type I polio virus (Mahoney strain) suspension were administered orally to six and eleven of cynomolgus monkeys in concentration of 1088.3 and 109.2 TCID50/ml respectivelly. One tothree of the infected monkeys were sacrificed (except 2 died cases) and autopsied every day during1-11 days. They were also observed on clinicalsigns, viremia and viral cultivation in feces beforethe autopsy.

 The indirect FAT method was chosen for themonkeys. Anti polio serum was obtained fromrabbit immunized with polio virus cultured in HeLacells. Anti rabbit-r-globulin goat serum combinedwith fluorescein isothiocyanate was used as the labeled antibody.

 The direct method was used for mice. Virus ofType II MEF-1 strain was cultured in HeLa cells. Serum from rabbits immunized with this virus wascombined with the dye above mentioned. The experiments of intracranial and intraspinal inoculationin mice were referred to the monkeys experiments. Viremia in monkeys was positive in 7 out of 15cases. Virus recovery in nasopharyngeal swabwas positive in 11 out of 15 cases.

 The lymph nodes, the liver and the spleen weretested by FAT. The viral antigen were found inthese tissues and organs of the monkeys.

 These findings revealed viral propagation in reticuloendothelial system in these tissues and organs. Moreover viral antigen was discovered in endothelial cells of tubules of the monkey kidney bymeans of FAT.

 In central nervous system, the viral antigen wasfound by FAT in cytoplasma of motoneurons in lumbar spinal grey matter of monkey autopsied 11days after virus oral administration.


Copyright © 1965, Igaku-Shoin Ltd. All rights reserved.

基本情報

電子版ISSN 1882-1243 印刷版ISSN 0001-8724 医学書院

関連文献

もっと見る

文献を共有