増殖糖尿病網膜症における酸化ストレスによる細胞傷害

Oxidative stress and cell damage in proliferative diabetic retinopathy

　背景：糖尿病網膜症の発症には酸化ストレスが関与している。目的：硝子体手術で得られた硝子体内の酸化ストレス物質の定量。対象：硝子体手術を行った44例46眼からの硝子体を対象とした。男性24例，女性20例で，年齢は61.6±10.2歳。内訳は増殖糖尿病網膜症18眼，網膜静脈分枝閉塞症による硝子体出血7眼，黄斑円孔13眼，黄斑上膜5眼，Terson症候群3眼である。糖尿病網膜症16眼には汎網膜光凝固が行われていた。方法：硝子体内の次の4項目を定量した。血管内皮増殖因子（VEGF），インターロイキン6（IL-6），一酸化窒素（NO）, 8-hydroxy-2'-deoxyguanosine（8-OHdG）。結果：4項目すべての値が，増殖糖尿病網膜症では他の群よりも有意に高かった。IL-6と8-OHdGとの間に有意な相関があった（r＝0.386，p＝0.0081）。結論：増殖糖尿病網膜症には酸化ストレスとサイトカインの関与がある。

　Background：Oxidative stress may be involved in the etiology of proliferative diabetic retinopathy. Purpose：To quantitate oxidative stress-related materials in human vitreous samples. Materials：Vitreous aspirates were obtained in 46 eyes of 44 patients during vitreous surgery. The series comprised 24males and 20 females. The ages averaged 61.6±10.2 years. The causative lesions were：proliferative diabetic retinopathy 18 eyes,vitreous hemorrhage secondary to branch retinal vein occlusion 7 eyes,and non-ischemic vitreoretinopathy 21 eyes including macular hole 13 eyes,epimacular membrane 5 eyes and Terson syndrome 3 eyes. Panretinal photocoagulation had been performed in 16 eyes of diabetic retinopathy. Following four items were quantitated：vascular endothelial growth factor（VEGF）,interleukin-6（IL-6）,8-hydroxy-2'-deoxyguanosine（8-OHdG）and nitrous oxide（NO）. Findings：Concentrations of all the four items were significantly higher in proliferative diabetic retinopathy than other diseases. The valur of IL-6 was significantly correlated with that of 8-OHdG（r＝0.386,p＝0.0081）. Conclusion：The findings show that oxidative stress and cytokines are involved in proliferative diabetic retinopathy.