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Brain and Nerve No to Shinkei Volume 42, Issue 10 （October 1990）

Brain and Nerve 脳と神経 42巻10号（1990年10月発行）

Japanese English

原著

ラマン分光法によるラットの脳水分含量の解析 ANALYSIS OF WATER CONTENT IN RAT BRAIN USING RAMAN SPECTROSCOPY 田渋公一 ¹ Koichi Tashibu ¹ ¹東京慈恵会医科大学脳神経外科 ¹Department of Neurosurgery, The Jikei University School of Medicine キーワード： laser Raman spectroscopy , braine dema , vasogenicedema , cytotoxic edema Keyword: laser Raman spectroscopy , braine dema , vasogenicedema , cytotoxic edema pp.999-1004

発行日 1990年10月1日 Published Date 1990/10/1

DOI https://doi.org/10.11477/mf.1406900116

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Abstract 文献概要
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　レーザーラマン分光法の脳組織への応用は未だほとんどなされていない。生体構成成分を細胞分子レベルで分析するにあたり，その精度の高さと簡便さとは今後脳神経外科領域においても多くが期待される。そこで今回，ラットを用いて，脳組織のCH基とOH基のラマンスペクトルを測定し，その分析を試み，脳浮腫に焦点を当てた。その結果，CH基は内部標準として使用可能であり，脳組織中の水分の相対含有量は3390と2935cm⁻¹のバンドの強度比のOH／CHで表わしうる事が判明した。脳皮質と視床ではOH／CHの値に有意差がなく灰白質と白質（橋）では灰白質のOH／CHの値が有意に高かった。乾燥標本では時間と共にOH／CH値の減少が見られ，有機錫による脳浮腫標本では視床の部分で，OH／CH値の著明な増加を認めたが，橋での変化は少なかった。

　今回の実験で細胞内にのみ浮腫液が貯留する有機錫によるcytotoxic edemaを簡便に，ほとんど侵襲もなく，しかも生態に近い状態で証明し得た。したがって，vasogenic edema，cytotoxic edemaの判別など，細胞レベルで脳浮腫の状態を分析し得る可能性が示された。

Laser Raman spectroscopy has not been applied to brain tissue. However, this method can pos-sibly analyze the constituent elements of living tissue of cellular and molecular level easily, and this might be essential for further brain research.

I attempted to analyze the water content of brain tissues, which was measured by Raman spec-trum of CH and OH groups, in normal and edema-tous rat brain tissues. The experimental results indicated that the peak intensity of CH group originated from protein, 2935 cm-1 in wave number, was constant in normal, dried and edematous brain tissue. Therefore it could be used as internal standard.

As the peak intensity of OH group, 3390 cm-1 in wave number, was originated from water, the relative amount of water content in the brain tissues could be evaluated by OH/CH value. The OH/CH value was not significantly different be-tween the cortex and the thalamus, but it was significantly higher in the gray matter (thalamus) than in the white matter (pons). The OH/CH value was decreased in dried specimens. In ex-perimental edematous brain tissues, which was produced by intraperitoneal injection of organic tin, the OH/CH value was evidently increased in the thalamus but not much changed in the pons. This results were well compatible with cytotoxic edema, which was reported to be produced by organic tin.

It is suggested that laser Raman spectroscopy can be used for brain edema research as one of the strong weapon, in analyzing water content of brain tissues on a cellular level.