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Japanese

ADOPTIVE IMMUNOTHERAPY FOR THE EXPERIMENTAL BRAIN TUMOR IN RATS:INDUCTION OF LAK CELLS AND THEIR BIOLOGICAL CHARACTERISTIC Nobuyuki Takai 1 , Ryuichi Tanaka 1 , Seiichi Yoshida 1 , Naoyuki Hara 1 , Takafumi Saito 1 1Department of Neurosurgery, Brain Research Institute, Niigata University pp.879-884
Published Date 1987/9/1
DOI https://doi.org/10.11477/mf.1406205975
  • Abstract
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We have investigated the biological characte-ristic of Lymphokine Activated Killer (LAK) cells induced from spleen cells (SPC) of Fischer rats.

Supernatant of 48 hour culture medium (RPMI-1640, 10% FBS, 2-ME : 5 × 10-5 M, HEPES : 10 mM) of SPC (1 x 106/ml) from Wistar rats in the presence of Con A (5 p.g/ml) was used as Inter-leukin 2 (IL-2). LAK cells were generated by co-cultivation of SPC (4 x 106/ml) from Fischer rats with peak reactivity on the 2 nd or 3 rd day of culture. Lytic activity was observed aginst not only syngeneic tumor cells (T9, RSV induced braintumor), but also allogenic (KMT-17) and xenogenic (K 562, Raj i cell, G 361, YAC-1) tumor cells, while no lytic activity was observed against normal brain cells. Cell depletion test, dye exclution test and immunofluorescence method using monoclonal Antibodies (mAbs) revealed that LAK cells partially belonged to the population of activated T cell group (W 3/13, OX 4 positive) but the precursor cells did not react with any mAbs used (W 3/13, W 3/25, OX 4, OX 8).

On the basis of these results, more effective and useful administration of LAK cells is now under investigations by using the rats with brain tumors.


Copyright © 1987, Igaku-Shoin Ltd. All rights reserved.

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電子版ISSN 2185-405X 印刷版ISSN 0006-8969 医学書院

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